, 2008 选用热变性法、离子交换层析技术和聚丙烯酰胺凝胶电泳技术(PAGE)小批量制备电泳纯魟鱼肝铁蛋白(Liverferritin ofDasyatis akajei,DALF)和黄牛胰脏铁蛋白(Scalper pancreas ferritin,SPF).等电聚胶电泳技术指出,DALF和SPF分别显示3和2条不同等电点铁蛋白层析带,推测这一现象与铁蛋白含铁量无关,与各自蛋白壳H和L亚基分布不同且形成不同表面电荷有关.不同等电点且相同含铁量的DALF,表现出不同的释放铁速率,其释放铁全过程均可分为快速和慢速阶段,
朱锋 +5 more
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Prognostic value of serum growth differentiation factor-15, brain natriuretic peptide and adiponectin in patients with acute myocardial infarction [PDF]
Objective To investigate the expression levels of serum growth differentiation factor-15 (GDF-15), brain natriuretic peptide (BNP), and adiponectin (APN) in patients with acute myocardial infarction (AMI) and their prognostic evaluation value.
GU Peipei*, WANG Haitao, WEI Xiaohui
core +2 more sources
基质辅助激光解吸电离飞行时间质谱技术研究人血清转铁蛋白稳定性及裂解产物 [PDF]
, 2007 制备质谱纯人血清转铁蛋白(HTF),供分子结构分析。选用SDS-PAGE、胶外酶解、基质辅助激光解吸/电离质谱技术(MALDI-TOF)、数据库检索和比对技术鉴定铁饱和HTF、双铁HTF(HTF-2Fe3+)、单铁HTF(HTF-Fe3+)和脱铁HTF(apoHTF)的稳定性和裂解产物。以乙腈溶液作为洗脱相,发现铁饱和HTF在RP-HPLC分离纯化过程中产生裂解现象。铁饱和HTF和HTF-2Fe3+经乙腈处理后均能产生不同分子量的短肽裂解产物,指出HTF结构稳定性与络合铁离子数量有关 ...
卓慧钦 +4 more
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Evaluation of early blood inflammation indicators on the prognosis of patients with severe community-acquired pneumonia [PDF]
Objective To evaluate the prognostic value of early blood inflammatory indicators in patients with respiratory failure caused by severe community-acquired pneumonia.
Sun Shiyu, Zhu Miao, Li Tiantian, Wang Haibo, Zuo Huaqin, Wang Xuejie
core +1 more source
Studies on the characters of mass spectrometry, electrophoresis and iron releasing kinetics of several one-kind-subunit ferritins [PDF]
, 2004 本文选用电泳和质谱等研究了三种由单类型亚基组成的铁蛋白的分子结构、组成和理化性质。作者采用制备电泳和离心超滤等技术，提取了电泳纯鲨鱼（Sphyrnazygaena）肝铁蛋白（LiverferritinofSphyrnazygaena，SZLF）、棕色固氮菌铁蛋白（Azotobactervinelandiiofbacterilaferritin,AVBF）和海兔肝铁蛋白（LiverferritinofAplysia，ALF），样品纯度适合于质谱和一级结构分析。SDS-聚丙烯酰胺凝胶电泳(SDS ...
陈旭
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Molecular Structure, Stability, and Novel Function of Human Serum Transferrin Revealed by MALDI-TOF Mass Spectrometry [PDF]
, 2007 转铁蛋白（transferrin,TF）是一类含有铁组分和糖链的金属蛋白质(80kDa)。TF的主要生理功能是输送铁给细胞，或作为生物体液的抗菌因子。TF有两个同源末端（C-和N-端），每个末端又分为两个结构域，结构域之间形成深裂（单一、高亲和力的铁结合位点）。本论文选用梯度、不连续天然凝胶电泳制备人血清转铁蛋白（HTF），并结合电转移和超滤技术进一步分离与浓缩HTF。运用SDS-PAGE、胶内与胶外酶解、MALDI-TOF质谱、数据库检索和比对法等分析技术鉴定HTF。HTF（77.1kDa ...
金宏伟
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[Correlation analysis between serum ferritin level and liver damage in acute stage of dengue fever]. [PDF]
Zhonghua Gan Zang Bing Za Zhi, 2021 Zhang XB +10 more
europepmc +1 more source
, 2019 肝细胞癌（HCC）是全球最常见的肝脏恶性肿瘤，肝切除、肝移植以及消融是早期HCC根治性的治疗方式。目前，我国大多数HCC患者确诊时已经处于疾病晚期，临床实践中以系统治疗为主。索拉非尼（sorafenib）和仑伐替尼（lenvatinib）作为国际公认的晚期HCC一线治疗方案。在亚洲地区，肝动脉灌注化疗（HAIC）也作为临床上晚期HCC的一线治疗；HAIC直接将抗肿瘤药物经导管注入至肝肿瘤供血血管，以获得较高的反应率和较明显的生存优势，降低了化疗药的全身毒副作用；然而 ...
赵明
core
[Research advances on the targeted programmed cell death regulatory network for treatment of refractory diabetic wounds]. [PDF]
Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za ZhiZhang ZH, He WF.
europepmc +1 more source
[Formononetin downregulates P53/SAT1/ACSL4 pathway-mediated ferroptosis to improve hypoxic-ischemic brain injury in neonatal mice]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoGuo T +8 more
europepmc +1 more source