HGF与CTLA-4Ig双基因修饰增强脐带间充质干细胞的免疫抑制和促增殖作用
【目的】 探讨人脐带间充质干细胞(hUCMSC)经肝细胞生长因子(HGF)和细胞毒性T细胞相关抗原4免疫球蛋白(CTLA4-Ig)双基因修饰后免疫调节能力的变化及其促进肝细胞增殖作用的影响。【方法】 酶联合消化法提取并鉴定人脐带间充质干细胞;对照组的hUCMSC转染携带绿色荧光蛋白(EGFP)基因的Ad5-EGFP,实验组的hUCMSC转染携带HGF和CTLA-Ig双基因的腺病毒Ad5-HGF/CTLA-4Ig。流式细胞仪检测对照组hUCMSC转染Ad5-EGFP后72 h表达EGFP细胞比率 ...
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Correlation between thrombus composition and regulatory T cell counts with clinical outcomes of acute ischemic stroke patients with thrombectomy. [PDF]
Luo J, Ma J.
europepmc +1 more source
[Overexpression of ubiquitin-conjugating enzyme 2T induces radiotherapy resistance in hepatocellular carcinoma by enriching regulatory T cells in the tumor microenvironment]. [PDF]
He X +5 more
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[Progress in regulating stem cell functions for repair and regeneration of craniomaxillofacial tissues]. [PDF]
Zhang H, Yang F, Yang R.
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FeSO4诱导PC12细胞MMP-9/MMP-2表达变化及对其凋亡的影响
目的探讨硫酸亚铁(FeSO4)诱导的脑出血(ICH)PC12细胞模型中基质金属蛋白酶-9(MMP-9)、基质金属蛋白酶-2(MMP-2)的表达变化,以及其对细胞氧化应激和凋亡的影响。方法复苏培养PC12细胞,随机分为ICH模型组和正常细胞组。采用MTT比色法检测两组细胞活力;Hoechst染色法观察细胞凋亡情况并计算凋亡率;比色法测定细胞内Caspase3、Caspase6、Caspase9活性;分光度法检测丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH ...
苑杨 +4 more
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[Research Progress of Peripheral Immune Tolerance in Oral Diseases]. [PDF]
Wen K, Zhang P, Peng X.
europepmc +1 more source
[The role and mechanism of chemokine network in promoting osteoarthritis progression by regulating synovial macrophage heterogeneity]. [PDF]
Lin Y, Wu L, Chen Q, Shen B.
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[Research progress on the multidimensional regulation of gut microbiota on metabolism, immunity, and inflammatory response in metabolic associated fatty liver disease]. [PDF]
Wu ST, Lu XL.
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[Research progress on protein lactylation modification in malignant tumors]. [PDF]
Dong H, Chen H, Zhang C.
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[Research Progress on the Biological Functions of Intestinal Eosinophils and Related Diseases]. [PDF]
Dou M, Zhang Y, Li M.
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