Results 11 to 20 of about 10,187 (206)
[Applications of high performance liquid chromatography-mass spectrometry in proteomics]. [PDF]
Se Pu蛋白质组学研究在生物医学领域发挥了重要作用,然而研究面临的主要难点在于其研究对象的复杂性和多样性。随着质谱技术的快速发展,高效液相色谱-质谱(HPLC-MS)分离分析复杂生物样品已经成为蛋白质组学研究的基础工具。蛋白质组学的研究从肽段分离,延伸到蛋白质和蛋白质复合物的分离,随着分析物的分子质量不断增大,其结构和组成复杂性也持续增加,分子特性也发生改变。面对不同的蛋白质组学研究对象,选择不同的分离模式、分离条件以及固定相参数是进行深度蛋白质组学研究的关键。本文综述了实验室常用的液相色谱分离模式 ...
Liu W, Weng LX, Gao MX, Zhang XM.
europepmc +2 more sources
[Advances in chiral separation and analysis by capillary electrophoresis-mass spectrometry]. [PDF]
Se Pu, 2022 目前使用的绝大多数药物为手性化合物,它们具有相似的物理和化学性质,但药理活性不同,且常以外消旋混合物的形式存在,因此对手性化合物的分离在生物、环境、食品和医药等领域一直备受关注。与广泛使用的液相色谱-质谱(LC-MS)相比,毛细管电泳-质谱(CE-MS)作为一种新型分离分析技术,具有分离效率高、样品和试剂消耗量低、选择性高和分离模式多样化等诸多优势,已经发展成为手性分析领域中有广阔应用前景的分析方法之一。CE-MS结合了CE的高分离效率和低样品消耗以及MS的高灵敏度和强结构解析能力 ...
Chi Z, Yang L.
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Integrating genomics and conservation to safeguard plant diversity 整合基因组学与保护生物学研究，保护植物多样性
Integrative Conservation, Volume 2, Issue 1, Page 10-18, March 2023., 2023 Abstract Conserving genetic diversity has been increasingly recognized as a keystone towards the protection of biodiversity and natural resources for future generations of humankind. In turn, the ongoing genomic revolution provides us with the technical innovations and expanded knowledge required to establish programs to integrate genomic approaches ...
Harald Schneider
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Journal of Diabetes, Volume 14, Issue 4, Page 271-281, April 2022., 2022 Highlights The gender‐specific role of sphingolipids in obesity‐related metabolic dysfunctions is unclear. The aim of this study is to identify ceramide species by liquid chromatography‐mass spectrometry that accumulate in central fat in relation to status of diabetes and gender for subjects with a body mass index ≥40 kg/m2 or > 35 kg/m2 with ...
Michelle Brusatori +7 more
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Veterinary Dermatology, Volume 32, Issue 5, Page 456-e124, October 2021., 2021 Background – Ceftazidime (CAZ) solutions are being used based on anecdotal reports for otitis externa complicated by multidrug‐resistant Pseudomonas aeruginosa (MDR PA). The chemical and microbiological stability of these proposed compounded solutions have not been evaluated, and likely are affected by the diluent and storage duration or temperature ...
Sarah E. Hoff +4 more
wiley +1 more source
[Application of chromatography-mass spectrometry in the clinical analysis of multiple sclerosis]. [PDF]
Se Pu多发性硬化（multiple sclerosis，MuS）是一种中枢神经系统慢性炎症和脱髓鞘疾病，全球患病率持续上升。其发病机制复杂，涉及免疫异常、遗传及环境因素，临床表现多样，在诊断与治疗方面面临挑战。代谢组学通过分析小分子代谢物，为揭示MuS病理机制和识别生物标志物开辟了新途径，尤其是在氨基酸和脂质代谢异常与炎症、髓鞘损伤及免疫反应关联的研究中发挥着重要作用。本文综述了色谱-质谱技术在MuS代谢标志物研究中的应用进展，聚焦氨基酸和脂质代谢的关键作用。回顾了液相色谱-串联质谱、高分辨质谱、气相色谱 ...
Yao ZY +5 more
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[Recent progress of chromatographic techniques for antibody purification]. [PDF]
Se Pu抗体作为一类重要的生物药物,在疾病诊断和治疗等方面拥有广阔的前景。抗体药物的需求逐年上升,生产规模不断扩大,下游抗体纯化已经成为抗体药物生产的瓶颈。色谱技术具有选择性好、分离效率高等优点,在抗体纯化领域占据主导地位。抗体分离纯化流程一般包括样品的粗提、粗提物精制和抗体精纯3个步骤,每个步骤都涉及色谱分离技术。目前已有多种色谱技术(如亲和色谱、离子交换色谱、疏水作用色谱、混合模式色谱以及新型的温敏色谱等)应用于抗体的分离纯化。本文介绍了近年来国内外各种色谱技术在抗体分离纯化方面所取得的研究进展 ...
Liu JW, Tang CW, Xia YR, Bai Q.
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The Oncologist, Volume 24, Issue 11, Page e1044-e1054, November 2019., 2019 Abstract Background The molecular phenotype of circulating tumor cells (CTCs) was associated with clinical outcome of patients with breast cancer. CTCs isolated from patients with metastatic breast cancer (MBC) display a unique microRNA (miRNA) expression profile.
Weige Tan +11 more
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Developmental Medicine &Child Neurology, Volume 61, Issue 3, Page E1-E35, March 2019., 2019 目的 本国际临床指南由欧洲残疾儿童学会（the European Academy of Childhood Disability，EACD）牵头制定，旨在解决发育性协调障碍（developmental coordination disorder，DCD）的定义、诊断、评估、干预以及与社会心理方面的临床应用关键问题。 方法 本指南针对五个领域的关键问题，通过文献综述和专家团队的正式讨论达成共识。为保证指南的循证基础，以“机制”、“评估”和“干预”为检索词, 对2012年更新以来提出的最新建议以及新增的“社会心理问题”和“青少年/成人”为检索词进行检索。根据牛津大学循证医学中心证据等级 (证据水平 [level of evidence, LOE]1–4) 将结果进行分类，最终转化为指南建议。并由国际 ...
Jing Hua +6 more
wiley +1 more source
Confocal Raman image method with maximum likelihood method [PDF]
, 2017 With the increasing interest in nano microscopic area, such as DNA sequencing, micro structure detection of molecular nano devices, a higher requirement for the spatial resolution of Raman spectroscopy is demanded.
Cui, Han +3 more
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