Results 111 to 120 of about 11,516 (168)
Transcriptomic analysis of the root system of Platycodon grandiflorum in response to the fallen leaves of Acer truncatum [PDF]
[Objective] The aim of this study was to explore the effect of Acer truncatum fallen leaves in Beijing on the accumulation of medicinal active ingredients of Platycodon grandiflorum and to reveal the molecular mechanism of active ingredient synthesis ...
CHENG Shaoying +5 more
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Zhongguo shiyan zhenduanxue, 2007 目的研究人脑转移瘤及瘤周围脑组织水通道蛋白的表达和调控特征,认识水通道蛋白的异常表达在脑水肿形成和消除中的作用。方法对5例肺腺癌脑转移病例经手术切除的肿瘤组织提取总RNA并进一步行RT-PCR反转录扩增明确脑转移瘤组织中水通道蛋白的表达类型,再对肿瘤组织病理切片行相应水通道蛋白的免疫组化染色,测量切片的灰度值。结果水通道蛋白4（AQP4）在脑转移瘤周围脑组织高表达,而在转移瘤组织内部未见表达。在瘤组织周围,距离瘤组织较远的区域AQP4染色较浅,紧贴瘤组织的区域AQP4染色显著加深 ...
刘军, 赵彬, 宿跃田, 刘丽丹
doaj
[EHHADH is a key gene in fatty acid metabolism pathways in hepatocellular carcinoma: a transcriptomic analysis]. [PDF]
Nan Fang Yi Ke Da Xue Xue Bao, 2023 Xie S, Li M, Jiang F, Yi Q, Yang W.
europepmc +1 more source
Study on factors affecting fruit development of seedless ponkan cultivar Huagan No.4 [PDF]
[Objective] Ponkan (Citrus reticulata Blanco.) is one of the main Mandarins cultivars in China. Huagan No.4 is a new seedless cultivar selected from a bud sport of Jing'an ponkan, with improved fruit quality. However, the cultivar has the problems of low
CHAI Lijun +11 more
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Zhongshan Daxue xuebao. Yixue kexue ban, 2017 摘要：【目的】探讨鼻咽癌异常表达的SOD1及其对细胞迁移能力的影响和可能的分子机制。【方法】采用免疫组化 技术检测癌和癌旁组织中SOD1的表达（n = 10），采用脂质体转染siRNA下调SOD1表达，用抑制剂LCS-1抑制SOD1，划痕 实验及迁移实验检测其对鼻咽癌细胞体外迁移能力的影响，定量PCR检测上皮细胞分子标志物E-cadherin及间质型分子 Vimentin及转录因子Twist的mRNA水平，Western Blot 法检测E-cadherin、Vimentin 蛋白水平。【结果 ...
付兰燕 +5 more
doaj
Genome-wide DNA methylation and transcriptome expression profiles of peripheral blood mononuclear cells in patients with systemic sclerosis with interstitial lung disease. [PDF]
Zhong Nan Da Xue Xue Bao Yi Xue Ban, 2023 Xie Y +5 more
europepmc +1 more source
The physiological mechanisms of key factors in IGF/ILP signaling system in the mud crab, Scylla paramamosain [PDF]
, 2017 胰岛素样生长因子（insulin-likegrowthfactor，IGF）／胰岛素样多肽（insulin-likepeptide，ILP）信号系统在脊椎动物的代谢和生长过程中起着至关重要的调控作用。近年来的研究表明，无脊椎动物中也分布着大量的ILP基因，这些基因及其编码的蛋白参与调控包括代谢、生长、生殖和蜕皮等在内的一系列生物学过程。迄今，有关甲壳动物IGF/ILP信号系统的组成、生物学功能及其信号传导途径的研究很少。 拟穴青蟹（Scyllaparamamosain ...
黄小帅
core
Zhongshan Daxue xuebao. Yixue kexue ban目的应用纳米孔长读长测序技术，深入分析正常肝实质细胞和肝癌细胞在低氧环境下的特异性转录组变化，以期阐明肿瘤细胞在低氧条件下生存与转移的内在机制，从而为肿瘤的精准靶向治疗开辟新的视角与策略。方法实验选取正常肝实质细胞系THLE-3与肝癌细胞系Hep3B作为研究对象，分别置于常氧与低氧条件下培养48 h后，提取其总RNA。随后，采用纳米孔测序技术，对这两种细胞系在不同氧浓度下的转录组进行高通量、高精度的测序分析。结果本研究成功构建了基于纳米孔三代测序技术的低氧转录本数据集，展现了前所未有的测序精度。通过GO（
高力, 沈晟齐, 高平
doaj +2 more sources
Zhongshan Daxue xuebao. Yixue kexue ban, 2010 【目的】 制备出一种人硫氧还蛋白(hTrx)基因修饰的肝细胞65377; 【方法】 逆转录聚合酶链反应法扩增出hTrx cDNA,应用重组逆转录病毒制备hTrx基因修饰大鼠肝细胞65377;白蛋白免疫组化SABC法进行肝细胞活性鉴定,MTT比色试验进行抗氧化应激能力检测65377;【结果】 克隆出hTrx开放阅读框cDNA并制备出重组逆转录病毒,感染真核细胞后可分泌融合表达的hTrx并具有生物学活性65377;制备出hTrx基因修饰大鼠肝细胞,免疫组化SABC法显示肝细胞功能正常 ...
doaj
[Application Progress of Dual RNA Sequencing in Microbial Research]. [PDF]
Sichuan Da Xue Xue Bao Yi Xue Ban, 2022 Xu MM, Sun YT, Chen H, Yang DQ.
europepmc +1 more source