小檗碱及其亲脂性衍生物抑制恶性胶质瘤C6细胞的增殖及迁移与侵袭
【目的】 考察小檗碱及其亲脂性衍生物对大鼠C6胶质瘤细胞增殖、迁移与侵袭的影响。【方法】 合成四种小檗碱衍生物9-O-十二烷基小檗碱(B3)、9-O-十六烷基小檗碱(B4)、9-O-十八烷基小檗碱(B5)、9-O-苄基小檗碱(B6)。采用1H NMR、13C NMR及ESI-MS确证其结构。四唑盐( MTT) 比色法检测化合物在不同浓度下作用24、48、72 h后对C6细胞增殖活性的影响; 细胞划痕实验和transwell迁移实验检测对细胞迁移能力的影响 ...
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