Results 61 to 70 of about 23,098 (190)
Research of Pfu polymerase improvement for Real-time fluorescent quantitative PCR [PDF]
, 2017 自从1988年Saiki等将耐热性的Taq聚合酶引入PCR反应后，极大地推动了PCR技术的推广，也带动了实时荧光定量PCR（qPCR）技术的兴起，分子生物学发展日新月异。随着技术的推广与应用，人们也逐渐发现了Taq聚合酶自身存在的缺点，其保真性差不适合高保真的PCR，复杂样本扩增能力差需要对样本进行处理以及体系的优化等等。而B族的Pfu聚合酶具有高保真性、高行进性和强耐干扰能力，但是不具有5’-3’外切活性且强3’-5’外切活性会水解游离探针影响荧光增益信号检测而不能用于qPCR ...
陆龙飞
core
Lessons learned from screening potential other effective area‐based conservation measures
Conservation Biology, EarlyView.Abstract Other effective area‐based conservation measures (OECMs) are sites that deliver effective biodiversity outcomes irrespective of their management objectives. These areas are widely expected to play an important role in efforts to protect 30% of Earth by 2030.
Carly N. Cook +15 more
wiley +1 more source
Gaoya dianqi, 2010 断路器在随机关合空载变压器时会产生幅值、频率很高的励磁涌流,这种操作暂态可能导致微机继保装置误动作,降低用户电能质量和系统可靠性。根据空载变压器涌流产生的机理,采用选相关合技术消除变压器励磁涌流,研究了铁心剩磁和最佳关合相位关系;分析了断路器截流和暂态恢复电压对铁心剩磁影响,并给出了剩磁测量方法。在空载变压器选相关合原理基础上,采用ATP-EMTP软件建立空载变压器关合模型,对暂态过程进行了仿真。仿真结果表明,选相关合技术能有效消除空载变压器合闸时产生的励磁涌流,为空载变压器选相关合提供了理论基础。
李伟 +4 more
doaj
Gaoya dianqi, 2023 随机合闸空载特高压换流变压器产生的励磁涌流容易造成合闸失败。文中分析了换流变合闸涌流的特殊性，并从理论上分析了剩磁、合闸角及合闸电阻对合闸涌流的影响，提出了合闸电阻结合选相合闸的特高压换流变压器励磁涌流抑制方法。利用PSCAD软件结合某±800 k V特高压直流换流站实际工况开展建模仿真，比较分析了合闸电阻、选相合闸及同时采用这两种措施抑制励磁涌流的效果。结果表明合闸电阻随机关合产生的励磁涌流高达额定电流的5.4倍；合闸电阻结合选相合闸抑制方法在保证选相合闸偏差时间在±2 ...
肖湃 +5 more
doaj
, 2017 田昭武,福建省福州市人,物理化学家,教育家,中国现代电化学奠基人和开拓者之一.田先生1927年6月28日出生于福建省福州市,1939年就读于福建省立永安中学直至完成初中、高中学业.优良的家庭教育和日本侵华的深重民族苦难,养成了他正直做人、勤奋做事、科学强国的坚定志向.1945年被保送到厦门大学化学系.在卢嘉锡、钱人元、蔡启瑞等名师熏陶和指导下 ...
万立骏, 孙世刚, 林昌健
core
An Image-Based Turbine Blade Parameter Inspection Method [PDF]
Accurate inspection of the feature parameters of the blade is a pivotal step to conduct the performance diagnosis and reverse modeling. To improve the inspection efficiency, an image-based parameter inspection method was proposed.
CUI Zeyang +3 more
core +1 more source
A link between increased temperature and avian body condition in a logged tropical forest
Conservation Biology, EarlyView.Abstract The combined effects of anthropogenic disturbances, such as logging and climate change, remain poorly understood; yet, they are the main threats to tropical biodiversity. Most tropical African countries lack long‐term climate data, so climate impacts on biodiversity cannot be assessed.
Moreen Uwimbabazi +7 more
wiley +1 more source
肾透明细胞癌差异表达基因的筛选及其核心基因相应miRNA和lncRNA 预测分析
Zhongshan Daxue xuebao. Yixue kexue ban, 2018 【目的】筛选肾透明细胞癌（ccRCC）中差异表达基因并其相关miRNA和lncRNA，寻找可作为ccRCC诊断及治疗的生物标志物。【方法】通过从TCGA数据库和GEO数据库筛选ccRCC差异表达基因并取交集，对差异表达基因进行GO功能分析和KEGG通路富集分析，使用MCODE软件筛选出差异表达基因中的核心基因，并利用mirDIP 数据库和STARBASE 数据库对核心基因上游的miRNA 和lncRNA 进行预测。【结果】共筛选出427个差异表达基因，这些差异表达基因在GO功能分析上主要与催化活性 ...
朱合欢 +5 more
doaj
Comprehensive framework for assessing and optimizing existing research networks
Conservation Biology, EarlyView.Abstract Conservation, monitoring, and research networks, or collections of ecological research sites unified under a common mission of data collection or a research mission, are essential infrastructure for understanding large landscapes. However, most networks developed opportunistically over decades rather than through systematic design, creating ...
Alyson East +2 more
wiley +1 more source
Gaoya dianqi, 2021 采用选相开关技术,可抑制电力系统暂态过程。实现断路器的位移按照给定曲线运动,降低合闸分散性,提高选相精度。传统的永磁式选相开关采用黑盒控制模型,存在控制器参数选取较困难、控制精度较低等问题。为此,建立永磁操纵机构内部数学模型,将电磁场与力场耦合,设计双闭环控制器。在Simulink中搭建仿真控制模型,改变外部电容电压,对比传统的黑盒控制模型,双闭环控制模型精度较高。以DSP28335为主控芯片,设计硬件控制系统,搭建实验平台,进行大量合闸控制实验。实验结果表明,采取上述的控制策略 ...
刘北阳 +4 more
doaj