Identification of apoptosis-related long non-coding RNAs expression profiles in patient with cervical intraepithelial neoplasia 3. [PDF]
Liu Z +5 more
europepmc +1 more source
目的应用多种分子标记,评估其在鉴定广州市花都区花山镇野鼠粪便中的广州管圆线虫的有效性,并分析野鼠的感染状况。方法捕获广州花都区花山镇128只野鼠,采集野鼠粪便,采用FastDNA SPIN Kit for Soil试剂盒提取粪便总DNA,通过PCR扩增获得cytb、cox I和ITS2片段,PCR产物经测序鉴定,并对测序产物进行系统发育分析。结果通过PCR扩增和测序鉴定,仅有5份样本扩增出的cytb序列没有套峰,可以准确鉴定为广州管圆线虫。结果表明该地区褐家鼠的广州管圆线虫感染率为7.58%(5/66),
吕进微 +4 more
doaj
[Expansion and identification of primary rat aortic vascular stem cells in vitro]. [PDF]
Ma H +5 more
europepmc +1 more source
[Isolation, identification and safety evaluation of <i>Lactobacillus gasseri</i> strain LGV03 isolated from the vagina of healthy women]. [PDF]
Su B +5 more
europepmc +1 more source
[Screening and functional identification of HLA-A*24:02-restricted HBsAg-specific TCR based on single-cell TCRαβ double-stranded amplification pairing]. [PDF]
Shen GJ +6 more
europepmc +1 more source
[Identification of chemical components of large-leaf yellow tea by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry]. [PDF]
Huang RT +6 more
europepmc +1 more source
丙型肝炎病毒基因高变区(E1,E2/NS1)的分子克隆及基因型鉴定
对广东省一个患者血清中的丙型肝炎病毒(HCV)基因高变区进行分子克隆及基因型鉴定。采用微粒吸附法提取HCVRNA,随机引物逆转录后进行聚合酶链反应(PCR)。所用引物位于E1,E2/NS1区,扩增产物806bp,产物提纯后定向插入pBV220质粒载体,重组体转染DH5α菌株,筛选菌落经增殖后碱变性法提取质粒,再通过PCR及酶切法鉴定阳性克隆。我们还用酶切法对此株克隆的基因型作了初步鉴定,证明属于1组Ⅱ型。
doaj
[Identification and Analysis of SND1 as an Oncogene and Prognostic Biomarker for Lung Adenocarcinoma]. [PDF]
Zhang R +9 more
europepmc +1 more source
Relationship between virulence and carbapenem resistance phenotype of Klebsiella pneumoniae from blood infection: identification of a carbapenem-resistant and hypervirulent strain. [PDF]
Liao Q +6 more
europepmc +1 more source

