摘 要: 【目的】 探讨微小RNA29a(miR-29a)在小鼠子宫内膜基质细胞(ESC)蜕膜化中的作用。【方法】 收集孕第1~9天(D1 ~ D9)的小鼠子宫,通过实时定量PCR(qRT-PCR)的方法检测小鼠子宫组织中miR-29a的表达;构建去除卵巢及其甾体激素的处理模型,观察甾体激素对miR-29a表达的影响。采用雌二醇(E2)和孕酮(P4)对分离培养的小鼠子宫内膜基质细胞进行蜕膜化处理,并通过瞬时转染的方法下调miR-29a的表达,分别收集蜕膜化处理不同时间及干扰前后的细胞,采用qRT ...
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