Results 21 to 30 of about 1,228,217 (166)
Rare Metals, Volume 45, Issue 2, February 2026.ABSTRACT This study introduces novel nonequiatomic TiZrCoCrMoCux (x = 0, 5, 10; at%) medium‐entropy alloys (MEAx) tailored for orthopedic implants, synergistically combining the biocompatibility of Ti‐based alloys, the wear resistance of CoCrMo alloys, and the antibacterial/osteogenic attributes of Cu. The alloy's microstructure, mechanical properties,
Hang Yu +9 more
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2019 【目的】评价医学外显子组高通量测序技术用于拷贝数变异（CNV）检测的可行性。【方法】两个不相关的遗传病家系案例，患者除了有智力障碍的类似症状外，无其他明显共同点。通过脆性X染色体综合征基因筛查、染色体G显带技术、染色体微阵列技术以及医学外显子组测序技术等对患者及其家属进行遗传学检测。【结果】在两个家系的患者中均发现染色体拷贝数变异，片段大小从11.4kb到13.03Mb不等。拷贝数变异得到了其他技术的验证。【结论】医学外显子组高通量测序技术用于拷贝数变异相关的遗传病临床辅助诊断是可行的 ...
张彦 +5 more
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Rare Metals, Volume 45, Issue 1, January 2026.ABSTRACT Thermodynamic prediction‐driven phase‐field kinetic simulation, with experimental verification, is utilized to explore the high‐performance cobalt–nickel based (CoNi‐based) superalloys. The roles of aluminum (Al) in microstructural evolution and precipitation kinetics of ordered L12‐γ′ strengthened Co–Ni–xAl superalloys are revealed. The alloy
Kun‐Ning Niu +6 more
wiley +1 more source
Integrative Conservation, Volume 4, Issue 4, Page 675-685, December 2025.We demonstrated that Anguilla japonica eDNA concentration was allometrically scaled with their body size in both the laboratory and riverine environments. Integrating body size allometry has the potential to improve population abundance estimates in the field, whereas further clarification is required for understanding eDNA production mechanisms ...
Toshiaki S. Jo +2 more
wiley +1 more source
野生动物学报, 2018 随着近年来高通量测序技术的发展,其在生物学研究领域的应用也越来越广泛,并逐渐成为生物学研究的重要方法和工具。基于高通量测序的 RNA-seq技术为揭示野生动物转录组信息提供了更为准确、便捷的方法。本文论述了RNA-seq技术的发展历史和技术特点,并结合野生动物转录组研究中所面临的问题探讨了RNA-seq技术的具体应用和发展方向,以期为野生动物的研究提供方法指导。
肖 慧 吴 尽 赵敏蝶 丁 新 刘学东
doaj
The Genome of Phoebe zhennan (Lauraceae) and Comparative Genomics of Phoebe 楠木(樟科)基因组与楠属植物比较基因组学研究
Integrative Conservation, Volume 4, Issue 4, Page 584-599, December 2025.Phoebe zhennan is a Chinese endemic and valuable timber species of Phoebe (Lauraceae). We present a chromosome‐level genome assembly of P. zhennan. Our research provides novel insights into the genome evolution of Phoebe, including whole‐genome duplication, phylogenetic relationships of magnoliids, and key MYB regulatory genes involved in lignin ...
Mao Yameng +3 more
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2007 【目的】获取人蛋白酪氨酸磷酸酯酶4A2（PTP4A2）基因并高效表达纯化,对于产物的体外活性进行测定。【方法】用RT-PCR技术,从肝癌细胞系HepG2的总RNA中,获得编码PTP4A2基因功能片段的DNA。测序后,通过酶切亚克隆至表达载体pGEX-4T-2,构建重组表达载体,并导入大肠杆菌E.coliBL21中,IPTG诱导表达重组的GST融合蛋白。对重组融合蛋白过谷胱甘肽琼脂糖柱进行纯化,western印记进行鉴定,质谱检测蛋白分子量。通过其对底物磷酸多肽的去磷酸化反应,鉴定其活性。【结果 ...
程超 +5 more
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Zhongshan Daxue xuebao. Yixue kexue ban, 2022 目的本研究通过第二代高通量测序（NGS）检测配对骨肉瘤转移灶和原发灶标本，分析骨肉瘤原发灶与转移灶的基因图谱差异，以期发现促进骨肉瘤发生和转移的相关分子及可能机制。方法12例转移患者的原发灶与转移病灶肿瘤组织样本，使用第二代高通量测序（NGS）进行检测，其中9对panel检测（678个基因）以及3对全外显子检测（WES），分析比较骨肉瘤原发灶与转移灶的基因图谱差异：基因拷贝数变异通过EXCAVATOR检测；DNA层面的融合突变通过Lumpy检测；RNA层面的融合突变通过Defuse + STAR ...
许明贤 +4 more
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Integrative Conservation, Volume 4, Issue 4, Page 665-674, December 2025.Using a diagnostic mitochondrial marker gene, we document the presence of furry‐eared dwarf lemurs, a species known from Madagascar's eastern montane rainforests, in several forest fragments across the island's central highlands. These findings raise new questions about when this species colonized these sites, how they tolerate these changing and ...
Marina B. Blanco +27 more
wiley +1 more source
Functional Ecology, Volume 39, Issue 12, Page 3504-3516, December 2025.Read the free Plain Language Summary for this article on the Journal blog. Abstract Nitrogen (N) deposition strongly affects litter decomposition and nutrient release, thereby reshaping carbon cycling in terrestrial ecosystems. Although most studies have focused on above‐ground litter, the effects of N addition on fine root decomposition and their ...
Yuneng Wang +5 more
wiley +1 more source