, 2008 本研究通过根癌农杆菌(Agrobacterium tumefaciens)介导，对多年生黑麦草进行遗传转化。用从成熟胚诱导的愈伤组织进行转化。经带有抗生素抗性基因的农杆菌侵染、抗生素筛选，获得抗性植株。经PCR、 GUS组织染色和Southern bloting验证，结果表明获得了稳定表达的转基因多年生黑麦草植株。该转化系统的建立，为定向进行分子设计改良黑麦草品种 ...
刘华玲, 杨宏, 马欣荣
core
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