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Evaluation of a Nanopore Sequencing Strategy on Bacterial Communities From Marine Sediments
Environmental DNAFollowing the development of high‐throughput DNA sequencers, environmental prokaryotic communities were usually described by metabarcoding on short markers of the 16S domain. Among third‐generation sequencers, that offered the possibility to sequence the
Alice Lemoinne +3 more
doaj +1 more source
A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins
FEBS Letters, EarlyView.We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue +6 more
wiley +1 more source
Deep learning for predicting 16S rRNA gene copy number
Scientific ReportsCulture-independent 16S rRNA gene metabarcoding is a commonly used method for microbiome profiling. To achieve more quantitative cell fraction estimates, it is important to account for the 16S rRNA gene copy number (hereafter 16S GCN) of different ...
Jiazheng Miao +3 more
doaj +1 more source
+16 or trisomy 16 (solely) [PDF]
Atlas of Genetics and Cytogenetics in Oncology and Haematology, 2011 Review on +16 or trisomy 16 (solely), with data on clinics, and the genes involved.
openaire +2 more sources
Seasonal variations in antibiotic resistance gene transport in the Almendares River, Havana, Cuba [PDF]
, 2012 Numerous studies have quantified antibiotic resistance genes (ARG) in rivers and streams around the world, and significant relationships have been shown that relate different pollutant outputs and increased local ARG levels.
Charles W Knapp +6 more
core +2 more sources
mSystems, 2015 We continue to uncover a wealth of information connecting microbes in important ways to human and environmental ecology. As our scientific knowledge and technical abilities improve, the tools used for microbiome surveys can be modified to improve the ...
William Walters +11 more
semanticscholar +1 more source
The IQ‐compete assay for measuring mitochondrial protein import efficiencies in living yeast cells
FEBS Letters, EarlyView.The efficiency of mitochondrial protein import depends on the properties of the newly synthesized precursor proteins. The Import and de‐Quenching Competition (IQ‐compete) assay is a novel method to monitor the import efficiency of different proteins by fluorescence in living yeast cells.
Yasmin Hoffman +3 more
wiley +1 more source
Bacterial Community Reconstruction Using A Single Sequencing Reaction [PDF]
, 2010 Bacteria are the unseen majority on our planet, with millions of species and comprising most of the living protoplasm. While current methods enable in-depth study of a small number of communities, a simple tool for breadth studies of bacterial population
Amir, Amnon, Zuk, Or
core
PLoS Biology, 2008 The human intestinal microbiota is essential to the health of the host and plays a role in nutrition, development, metabolism, pathogen resistance, and regulation of immune responses. Antibiotics may disrupt these coevolved interactions, leading to acute
L. Dethlefsen +3 more
semanticscholar +1 more source
FEBS Letters, EarlyView.Anaplastic thyroid cancer (ATC) lacks iodide uptake ability due to MAPK activation increasing the expression of the histone methyltransferase EZH2, which represses thyroid differentiation genes (TDGs) such as the sodium iodide symporter (NIS). Dual inhibition of MAPK (U0126) and EZH2 (EPZ6438/Tazemetostat) reverses this mechanism, thus restoring TDG ...
Diego Claro de Mello +6 more
wiley +1 more source