Results 131 to 140 of about 712,979 (385)

Characterization of the Gut Microbiome Using 16S or Shotgun Metagenomics

Frontiers in Microbiology, 2016
The advent of next generation sequencing (NGS) has enabled investigations of the gut microbiome with unprecedented resolution and throughput. This has stimulated the development of sophisticated bioinformatics tools to analyze the massive amounts of data
J. Jovel, Jordan Patterson, Weiwei Wang, N. Hotte, S. O’keefe, Troy Mitchel, T. Perry, D. Kao, A. Mason, K. Madsen, G. Wong   +10 more
semanticscholar   +1 more source

Thermostable neutral metalloprotease from Geobacillus sp. EA1 does not share thermolysin's preference for substrates with leucine at the P1′ position

FEBS Letters, EarlyView.
Knowing how proteases recognise preferred substrates facilitates matching proteases to applications. The S1′ pocket of protease EA1 directs cleavage to the N‐terminal side of hydrophobic residues, particularly leucine. The S1′ pocket of thermolysin differs from EA's at only one position (leucine in place of phenylalanine), which decreases cleavage ...
Grant R. Broomfield, Torsten Kleffmann, David J. Saul, Sigurd M. Wilbanks   +3 more
wiley   +1 more source

Evidence for diversifying selection of genetic regions of encoding putative collagen-like host-adhesive fibers in Pasteuria penetrans [PDF]

, 2019
© FEMS 2018. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided ...
Anderson, Andrews, Arohi Srivastava, Arslan, Atibalentja, Aziz, Carver, Charles, Cohan, Colson, Davies, Davies, Davies, Davies, Davies, Davies, Degnan, DePristo, Duan, Dutta, Eardly, Espanol, Felsenstein, Filée, Gascuel, Giblin-Davis, Giblin-Davis, Huynen, Jones, Kanehisa, Kaufman, Keith G Davies, Kumar, Legendre, Luijckx, Luijckx, Maddison, Mauchline, McElroy, Metchnikoff, Mohan, Mohan, Mouton, Murtagh, Noel, Philippe, R Developement Core Team, Radó-Trilla, Rajendhran, Romero, Rutherford, Sayre, Sayre, Schouls, Sharad Mohan, Sharma, Stackebrandt, Starr, Steichen, Stirling, Stirling, Sturhan, Sylvestre, Sylvestre, Tamura, Tim H Mauchline, Todd, Wiedenbeck, Woese, Yap, Zilversmit   +70 more
core   +3 more sources

An improved dual-indexing approach for multiplexed 16S rRNA gene sequencing on the Illumina MiSeq platform

Microbiome, 2014
To take advantage of affordable high-throughput next-generation sequencing technologies to characterize microbial community composition often requires the development of improved methods to overcome technical limitations inherent to the sequencing ...
D. Fadrosh, Bing Ma, P. Gajer, Naomi A. Sengamalay, Sandra H Ott, R. Brotman, J. Ravel   +6 more
semanticscholar   +1 more source

Mechanisms and kinetic assays of aminoacyl‐tRNA synthetases

FEBS Letters, EarlyView.
Igor Zivkovic, Morana Dulic, Ita Gruic‐Sovulj   +2 more
wiley   +1 more source

Neutrophil deficiency increases T cell numbers at the site of tissue injury in mice

FEBS Letters, EarlyView.
In wild‐type mice, injury or acute inflammation induces neutrophil influx followed by macrophage accumulation. Mcl1ΔMyelo (neutrophil‐deficient) mice lack neutrophils, and in response to muscle injury show fewer macrophages and exhibit strikingly elevated T‐cell numbers, primarily non‐conventional “double‐negative” (DN) αβ and γδ T cells.
Hajnalka Halász, Albert Bálint Papp, Lukács Sándor Lesinszki, Attila Mócsai, Tamás Varga, László Nagy, Péter Gogolák   +6 more
wiley   +1 more source

Bacterial genotyping by 16S rRNA mass cataloging

BMC Bioinformatics, 2006
Background It has recently been demonstrated that organism identifications can be recovered from mass spectra using various methods including base-specific fragmentation of nucleic acids.
Fox George E, McNichols Roger J, Jackson George W, Willson Richard C   +3 more
doaj   +1 more source

Ingots and ingot moulds. By A. W. and H. Brearley. Pp. xv + 218. (London: Longmans, Green and Co. 1918.) Price 16s. net [PDF]

, 1918
C. O. Bannister
openalex   +1 more source

Accuracy of taxonomy prediction for 16S rRNA and fungal ITS sequences

PeerJ, 2018
Prediction of taxonomy for marker gene sequences such as 16S ribosomal RNA (rRNA) is a fundamental task in microbiology. Most experimentally observed sequences are diverged from reference sequences of authoritatively named organisms, creating a challenge
Robert C. Edgar
semanticscholar   +1 more source

Development of visible light‐sensitive human neuropsin (OPN5) via single amino acid substitution

FEBS Letters, EarlyView.
The present study determines a key amino acid residue, Lys91, for defining UV sensitivity of human OPN5. Heterologous action spectroscopy of the wild type and K91 mutants of OPN5 in HEK293T cells reveals that substitution of Lys91 with neutral (alanine) or acidic amino acids (glutamic or aspartic acids) causes substantial shifts in spectral sensitivity
Yusuke Sakai, Richard J. McDowell, Robert J. Lucas   +2 more
wiley   +1 more source