Results 131 to 140 of about 736,237 (386)

16S rRNA gene sequencing of mock microbial populations- impact of DNA extraction method, primer choice and sequencing platform [PDF]

, 2016
peer-reviewedBackground Next-generation sequencing platforms have revolutionised our ability to investigate the microbiota composition of complex environments, frequently through 16S rRNA gene sequencing of the bacterial component of the
Claesson, Marcus J., Clooney, Adam G, Cotter, Paul D., Fouhy, Fiona, STANTON, CATHERINE   +4 more
core   +2 more sources

The effect of 16S rRNA region choice on bacterial community metabarcoding results

Scientific Data, 2019
In this work, we compare the resolution of V2-V3 and V3-V4 16S rRNA regions for the purposes of estimating microbial community diversity using paired-end Illumina MiSeq reads, and show that the fragment, including V2 and V3 regions, has higher resolution
Y. Bukin, Y. Galachyants, I. V. Morozov, S. Bukin, A. Zakharenko, T. Zemskaya   +5 more
semanticscholar   +1 more source

By dawn or dusk—how circadian timing rewrites bacterial infection outcomes

FEBS Letters, EarlyView.
The circadian clock shapes immune function, yet its influence on infection outcomes is only beginning to be understood. This review highlights how circadian timing alters host responses to the bacterial pathogens Salmonella enterica, Listeria monocytogenes, and Streptococcus pneumoniae revealing that the effectiveness of immune defense depends not only
Devons Mo, Catherine S. Palmer, Jacqueline M. Kimmey   +2 more
wiley   +1 more source

Hematopoietic (stem) cells—The elixir of life?

FEBS Letters, EarlyView.
The aging of HSCs (hematopoietic stem cells) and the blood system leads to the decline of other organs. Rejuvenating aged HSCs improves the function of the blood system, slowing the aging of the heart, kidney, brain, and liver, and the occurrence of age‐related diseases.
Emilie L. Cerezo, Jonah Anderson, Emilie Dinh Vedrenne, Noël Yeh Martín, Jette Lengefeld   +4 more
wiley   +1 more source

Bacterial genotyping by 16S rRNA mass cataloging

BMC Bioinformatics, 2006
Background It has recently been demonstrated that organism identifications can be recovered from mass spectra using various methods including base-specific fragmentation of nucleic acids.
Fox George E, McNichols Roger J, Jackson George W, Willson Richard C   +3 more
doaj   +1 more source

Exploring soil microbial 16S rRNA sequence data to increase carbon yield and nitrogen efficiency of a bioenergy crop [PDF]

, 2015
Leonardo Machado Pitombo, Janaína Braga do Carmo, Mattias de Hollander, Raffaella Rossetto, Maryeimy Varon Lopez, Heitor Cantarella, Eiko E. Kuramae   +6 more
openalex   +1 more source

Characterization of the Gut Microbiome Using 16S or Shotgun Metagenomics

Frontiers in Microbiology, 2016
The advent of next generation sequencing (NGS) has enabled investigations of the gut microbiome with unprecedented resolution and throughput. This has stimulated the development of sophisticated bioinformatics tools to analyze the massive amounts of data
J. Jovel, Jordan Patterson, Weiwei Wang, N. Hotte, S. O’keefe, Troy Mitchel, T. Perry, D. Kao, A. Mason, K. Madsen, G. Wong   +10 more
semanticscholar   +1 more source

Structural insights into lacto‐N‐biose I recognition by a family 32 carbohydrate‐binding module from Bifidobacterium bifidum

FEBS Letters, EarlyView.
Bifidobacterium bifidum establishes symbiosis with infants by metabolizing lacto‐N‐biose I (LNB) from human milk oligosaccharides (HMOs). The extracellular multidomain enzyme LnbB drives this process, releasing LNB via its catalytic glycoside hydrolase family 20 (GH20) lacto‐N‐biosidase domain.
Xinzhe Zhang, Naoki Sunagawa, Toma Kashima, Kiyohiko Igarashi, Akimasa Miyanaga, Shinya Fushinobu   +5 more
wiley   +1 more source

A Simple Microbiome in the European Common Cuttlefish, Sepia officinalis. [PDF]

, 2019
The European common cuttlefish, Sepia officinalis, is used extensively in biological and biomedical research, yet its microbiome remains poorly characterized.
Abbo, Lisa, Durand, Amber, Gilbert, Jack A, Gottel, Neil R, Hanlon, Roger T, Lutz, Holly L, Mark Welch, Jessica L, Ramírez-Puebla, S Tabita, Schlundt, Cathleen, Sjaarda, Alexandra K   +9 more
core   +1 more source

An improved dual-indexing approach for multiplexed 16S rRNA gene sequencing on the Illumina MiSeq platform

Microbiome, 2014
To take advantage of affordable high-throughput next-generation sequencing technologies to characterize microbial community composition often requires the development of improved methods to overcome technical limitations inherent to the sequencing ...
D. Fadrosh, Bing Ma, P. Gajer, Naomi A. Sengamalay, Sandra H Ott, R. Brotman, J. Ravel   +6 more
semanticscholar   +1 more source