Background High-throughput sequencing of bacterial 16S rRNA gene (16S-seq) is a useful and common method for studying bacterial community structures. However, contamination of the 16S rRNA genes from the mitochondrion and plastid hinders the sensitive ...
Luyang Song, Kabin Xie
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Utilization of Pyrosequencing to Monitor the Microbiome Dynamics of Probiotic Treated Poultry (Gallus gallus domesticus) during Downstream Poultry Processing [PDF]
Antibiotic growth promoters that have been historically employed to control pathogens and increase the rate of animal development for human consumption are currently banned in many countries.
Bray, Joey +3 more
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PHYLOGENY OF SOME MIDDLE AMERICAN PITVIPERS BASED ON A CLADISTIC ANALYSIS OF MITOCHONDRIAL 12S AND 16S DNA SEQUENCE INFORMATION [PDF]
The cladistic relationships of several Middle American pitvipers representing the genera Bothrops (sensu stricto), Bothriechis, Cerrophidion, Lachesis and Porthidium were determined using mitochondrial 12S and 16S DNA sequence information.
Crother, Brian I. +2 more
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Increased variability of microbial communities in restored salt marshes nearly 30 years after tidal flow restoration [PDF]
We analyzed microbial diversity and community composition from four salt marsh sites that were impounded for 40–50 years and subsequently restored and four unimpounded sites in southeastern Connecticut over one growing season.
Bernhard, Anne E +2 more
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Conservative fragments in bacterial 16S rRNA genes and primer design for 16S ribosomal DNA amplicons in metagenomic studies. [PDF]
Bacterial 16S ribosomal DNA (rDNA) amplicons have been widely used in the classification of uncultured bacteria inhabiting environmental niches. Primers targeting conservative regions of the rDNAs are used to generate amplicons of variant regions that ...
Yong Wang, Pei-Yuan Qian
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Nitrogen removal in a two-chambered microbial fuel cell: Establishment of a nitrifying-denitrifying microbial community on an intermittent aerated cathode [PDF]
A microbial fuel cell (MFC) was used to study nitrogen dynamics and its feasibility for high strength wastewater treatment. Intermittent aeration was applied on the cathode chamber accomplishing the establishment of a simultaneous nitrifying-denitrifying
Bonmatí Blasi, August +3 more
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Microhyla ornata, a species originally described from the southwest coast of India in 1841, was long reported to be wide-ranging throughout South, Southeast, and East Asia. Although the name M.
Sonali Garg +3 more
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Microbial Composition and Diversity of High-demand Street-vended Foods in Ecuador
Developing countries such as Ecuador carry a heavy food safety burden but reports on the microbiological quality of their foods are scarce. In this investigation, the microbial diversity of 10 high-risk and mass-consumption street-vended foods including ...
Byron Díaz Cárdenas +6 more
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OTU analysis using metagenomic shotgun sequencing data. [PDF]
Because of technological limitations, the primer and amplification biases in targeted sequencing of 16S rRNA genes have veiled the true microbial diversity underlying environmental samples. However, the protocol of metagenomic shotgun sequencing provides
Xiaolin Hao, Ting Chen
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Variable Region Sequences Influence 16S rRNA Performance
16S rRNA gene sequences are commonly analyzed for taxonomic and phylogenetic studies because they contain variable regions that can help distinguish different genera. However, intra-genus distinction using variable region homology is often impossible due
Nikhil Bose, Sean D. Moore
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