Results 21 to 30 of about 736,237 (386)

Jumping the green wall: The use of PNA‐DNA clamps to enhance microbiome sampling depth in wildlife microbiome research

Ecology and Evolution, 2020
As microbiome research moves away from model organisms to wildlife, new challenges for microbiome high‐throughput sequencing arise caused by the variety of wildlife diets.
Luis Víquez‐R, Ramona Fleischer, Kerstin Wilhelm, Marco Tschapka, Simone Sommer   +4 more
doaj   +1 more source

Novel hemotropic mycoplasmas are widespread and genetically diverse in vampire bats [PDF]

, 2017
Bats (Order: Chiroptera) have been widely studied as reservoir hosts for viruses of concern for human and animal health. However, whether bats are equally competent hosts of non-viral pathogens such as bacteria remains an important open question. Here,
Altizer, S.M., Becker, D.J., Bergner, L.M., Camus, M.S., Chizhikov, V.E., Orton, R.J., Streicker, D.G., Volokhov, D.V.   +7 more
core   +1 more source

Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies

Nucleic Acids Research, 2012
16S ribosomal RNA gene (rDNA) amplicon analysis remains the standard approach for the cultivation-independent investigation of microbial diversity. The accuracy of these analyses depends strongly on the choice of primers.
A. Klindworth, E. Pruesse, Timmy Schweer, J. Peplies, Christian Quast, M. Horn, F. Glöckner   +6 more
semanticscholar   +1 more source

Implementación de una estrategia de espectrometría de doble masa MALDI TOT/TOF para la identificación molecular de bacterias del intestino de trips del banano

Manglar, 2018
Los insectos plaga, conocidos como trips del banano (Chaetanaphothrips signipennis y Frankliniella parvula), vienen causando serios daños económicos al sector bananero de la costa norte del Perú.
Néstor Díaz-Castillo, Dicson Sánchez, Mercedes Oyola, Pedro Masías, R. García-Seminario, Virna Cedeño, Eric Mialhe   +6 more
doaj   +1 more source

Primer selection impacts specific population abundances but not community dynamics in a monthly time-series 16S rRNA gene amplicon analysis of coastal marine bacterioplankton. [PDF]

, 2018
Primers targeting the 16S small subunit ribosomal RNA marker gene, used to characterize bacterial and archaeal communities, have recently been re-evaluated for marine planktonic habitats.
Carlson, Craig A, Nelson, Craig E, Wear, Emma K, Wilbanks, Elizabeth G   +3 more
core   +1 more source

Diversity of culturable moderately halophilic and halotolerant bacteria in a marsh and two salterns a protected ecosystem of Lower Loukkos (Morocco) [PDF]

, 2012
To study the biodiversity of halophilic bacteria in a protected wetland located in Loukkos (Northwest, Morocco), a total of 124 strains were recovered from sediment samples from a marsh and salterns.
Amar, Mohamed, Bendaou, Najib, Berrada, Imane, De Vos, Paul, El Fahime, ElMostafa, Melloul, Marouane, Swings, Jean, Willems, Anne   +7 more
core   +1 more source

Phylogenies of the 16S rRNA gene and its hypervariable regions lack concordance with core genome phylogenies

Microbiome, 2022
Background The 16S rRNA gene is used extensively in bacterial phylogenetics, in species delineation, and now widely in microbiome studies. However, the gene suffers from intragenomic heterogeneity, and reports of recombination and an unreliable ...
H. Hassler, Brett Probert, Carson Moore, Elizabeth Lawson, Richard W. Jackson, Brook T. Russell, V. P. Richards   +6 more
semanticscholar   +1 more source

Long-read MinION™ sequencing of 16S and 16S-ITS-23S rRNA genes provides species-level resolution of Lactobacillaceae in mixed communities

Frontiers in Microbiology, 2023
The Lactobacillaceae are lactic acid bacteria harnessed to deliver important outcomes across numerous industries, and their unambiguous, species-level identification from mixed community environments is an important endeavor.
Sandra A. Olivier, Sandra A. Olivier, Michelle K. Bull, Michelle K. Bull, Mikael Lenz Strube, Robert Murphy, Tom Ross, John P. Bowman, Belinda Chapman, Belinda Chapman   +9 more
doaj   +1 more source

Reconciliation between operational taxonomic units and species boundaries [PDF]

, 2017
The development of high-throughput sequencing technologies has revolutionised the field of microbial ecology via 16S rRNA gene amplicon sequencing approaches.
Boon, Nico, Kerckhof, Frederiek-Maarten, Leys, Natalie, Monsieurs, Pieter, Mysara, Mohamed, Props, Ruben, Raes, Jeroen, Vandamme, Peter   +7 more
core   +2 more sources

Strategy for the identification of micro-organisms producing food and feed products : bacteria producing food enzymes as study case [PDF]

, 2020
Recent European regulations require safety assessments of food enzymes (FE) before their commercialization. FE are mainly produced by micro-organisms, whose viable strains nor associated DNA can be present in the final products.
De Keersmaecker, Sigrid C. J., Deckers, Marie, Deforce, Dieter, Denayer, Sarah, Fraiture, Marie-Alice, Heyndrickx, Marc, Roosens, Nancy H. C., Vanneste, Kevin, Winand, Raf   +8 more
core   +2 more sources