Results 61 to 70 of about 736,237 (386)

Reducing the Effects of PCR Amplification and Sequencing Artifacts on 16S rRNA-Based Studies

open access: yesPLoS ONE, 2011
The advent of next generation sequencing has coincided with a growth in interest in using these approaches to better understand the role of the structure and function of the microbial communities in human, animal, and environmental health.
P. Schloss, D. Gevers, Sarah L. Westcott
semanticscholar   +1 more source

Clinical Correlates of Anxiety and Depression After Diagnosis of a Pediatric Brain Tumor

open access: yesPediatric Blood &Cancer, EarlyView.
ABSTRACT Background The prevalence and clinical correlates of symptoms of anxiety and depression in pediatric patients with brain tumors are not well described. We aimed to identify clinical characteristics that are correlated with elevated symptoms of anxiety and depression following initial diagnosis.
Bryony J. Lucas   +16 more
wiley   +1 more source

Preoperative Cytopenia in Patients Affected by High‐Risk Neuroblastoma: Just a Matter of Platelets?

open access: yesPediatric Blood &Cancer, EarlyView.
ABSTRACT Background and Aims In patients affected by high‐risk neuroblastoma (HR‐NB), complete macroscopic resection (CMR) is associated with better outcomes. These patients are often cytopenic due to intensive induction regimens. The aim of the present study is to assess the impact of preoperative cytopenia on surgical outcome in patients with HR‐NB ...
Giorgio Persano   +13 more
wiley   +1 more source

Combination of 16S rRNA and procalcitonin in diagnosis of neonatal clinically suspected sepsis

open access: yesJournal of International Medical Research, 2020
Objective To investigate the application of 16S rRNA in diagnosing patients with neonatal sepsis. Methods We studied 60 consecutive neonatal patients with clinically suspected sepsis and 20 non-infective cases as controls.
Renqiang Yu   +4 more
doaj   +1 more source

Trends of the major porin gene (ompF) evolution [PDF]

open access: yes, 2011
OmpF is one of the major general porins of Enterobacteriaceae that belongs to the first line of bacterial defense and interactions with the biotic as well as abiotic environments.
Isaeva, Marina P.   +4 more
core   +1 more source

ampvis2: an R package to analyse and visualise 16S rRNA amplicon data

open access: yesbioRxiv, 2018
Summary Microbial community analysis using 16S rRNA gene amplicon sequencing is the backbone of many microbial ecology studies. Several approaches and pipelines exist for processing the raw data generated through DNA sequencing and convert the data into ...
K. S. Andersen   +3 more
semanticscholar   +1 more source

DELP Treatment on Vision and Retinal Microcirculation in Patients With Acute Ischemic Stroke: Report of Five Cases and Literature Review

open access: yesTherapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Background The delipid extracorporeal lipoprotein filter from plasma (DELP) treatment can effectively reduce blood lipid, increase blood flow, and improve neurological deficits in patients with acute ischemic stroke (AIS). However, its effect on vision and retinal microcirculation in stroke patients has never been reported.
Ning Li   +9 more
wiley   +1 more source

Molecular cloning of the rRNA genes of Prevotella bryantii B14

open access: yesActa Agriculturae Slovenica, 2000
Parts of the rRNA operons of anaerobic rumen bacteria Prevotella bryantii B14 were cloned. 350 colonies carrying pBluescriptII with HindIII DNA fragments were screened with 16S rDNA specific probe and six positive clones were obtained.
Matjaž PETERKA, Gorazd AVGUŠTIN
doaj   +1 more source

Latitudinal variation in the potential activity of Atlantic Ocean bacterioplankton revealed through 16S rRNA and 16S rRNA gene metabarcoding

open access: yesFrontiers in Marine Science, 2023
The activities of bacterioplankton sustain open ocean biogeochemical and ecological processes, however, little is known about the activity of specific bacterioplankton, especially related to their biogeography across oceanic scales.
Ro Allen   +5 more
doaj   +1 more source

Tax4Fun: predicting functional profiles from metagenomic 16S rRNA data

open access: yesBioinform., 2015
Motivation: The characterization of phylogenetic and functional diversity is a key element in the analysis of microbial communities. Amplicon-based sequencing of marker genes, such as 16S rRNA, is a powerful tool for assessing and comparing the structure
K. Aßhauer   +3 more
semanticscholar   +1 more source

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