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Characteristic archaebacterial 16S rRNA oligonucleotides
Systematic and Applied Microbiology, 1986A method of analyzing 16S rRNA catalog data has been developed in which groupings at various taxonomic levels can be characterized in terms of specific "signature" oligonucleotides. This approach provides an alternative means for evaluating higher order branching possibilities and can be used to assess the phylogenetic position of isolates that are ...
Mark H. Pickett+5 more
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The hypothesized role of YbeZ in 16S rRNA maturation
Archives of Microbiology, 2022Ribosomes are the protein production machines in all living cells. Yet in contrast to our understanding of how the ribosome translates DNA information into life, the steps involved in ribosome biogenesis, the assembly of the ribosomal RNA (rRNA) and protein molecules that make up the ribosome, remain incomplete.
Emma S. V. Andrews, Wayne M. Patrick
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The diversity of periodontal spirochetes by 16S rRNA analysis
Oral Microbiology and Immunology, 2000The purpose of this study was to examine the diversity of spirochetes in the subgingival pocket of multiple subjects with a range of periodontal conditions, including two healthy, one adult periodontitis, three acute necrotizing ulcerative gingivitis, eight refractory periodontitis, and one human immunodeficiency virus (HIV) periodontitis. The 16S rRNA
Bruce J. Paster+8 more
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16S rRNA sequencing in chronic dacryocystitis
Clinical and Experimental OptometryThe pathogenesis of chronic dacryocystitis (CDC) is associated with a variety of bacteria. Investigating microflora has the potential to provide a theoretical basis for preventing and treating CDC.16S rRNA sequencing is a sequence-based bacterial analysis. The application of 16S rRNA sequencing in CDC is rarely reported.A case-control study of infected
Yongxin, Zhang+7 more
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The role of 16S rRNA in ribosomal binding of IF-3
Biochemistry, 1976The binding of initiation factor IF-3 to Escherichia coli 30S ribosomal subunits has been found to be inhibited by rRNA ligands such as ethidium bromide, polyamines, and monovalent alkali metals. The order of effectiveness of the polyamines (spermine greater than spermidine greater than putrescine) and alkali metals (Li+ greater than Na+ greater than K+
Claudio O. Gualerzi, Cynthia L. Pon
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16S rRNA Amplicon Sequencing for Metagenomics
2018The 16S rRNA amplicon sequencing technique is a microbiome analysis where different samples are analyzed at the same time using multiplexing. The results can be used to evaluate microbial diversity at genus, family, order, class, and phylum levels. The resolution is normally insufficient to evaluate the species level.
Steffen Jørgensen+3 more
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Specific protection of 16 S rRNA by translationalinitiation factors
Journal of Molecular Biology, 1995The binding of initiation factors to 30 S ribosomal subunits protects specific sets of nucleotides in 16 S rRNA from base-specific chemical probes. Initiation factor 3 (IF-3) protects residues G700, G703 and G791 from attack by kethoxal. These protected bases are close to those in 16 S rRNA that are protected by 50 S subunits, providing a structural ...
Danesh Moazed+3 more
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rRNA topography in ribosome. IV. The accessibility of the 5'-end region of 16S rRNA
Molecular Biology Reports, 1979The accessibility of the 5'-end region of 16S rRNA (A8GAGUUUG15) inEscherichia coli ribosomes for complementary binding with the synthetic octanucleotide d(CAAACTCT) has been studied. Nonequilibrium gel-filtration was used to evaluate parameters of the binding of this oligonucleotide with free 16S rRNA, ribosomal subunits, and 70S ribosomes.
Alexey Kopylov+4 more
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A procedure for the preparation of rRNA from Legionellaceae for use in 16S-rRNA sequencing
Journal of Microbiological Methods, 1990Abstract rRNA-sequence comparison has become a commonly used method in bacterial taxonomy. To obtain intact rRNA from legioneallae for use in sequencing a simple enzymatic procedure was developed with requires no specialized equipment for release of RNA and no ultracentrifugation for its isolation. RNase inhibitors were included at each step to avoid
Arnold Brown, Karen F. Fox
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Journal of Biomolecular Structure and Dynamics, 1988
Interactions between RNA molecules have been postulated to play an important role in the assembly of ribosomes. Using the sequence analysis and the search of continuous complementary regions on 16S rRNA and 23S rRNA, the recognition sites involved in the formation of ribosome of E. coli are postulated.
M W Pandit+2 more
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Interactions between RNA molecules have been postulated to play an important role in the assembly of ribosomes. Using the sequence analysis and the search of continuous complementary regions on 16S rRNA and 23S rRNA, the recognition sites involved in the formation of ribosome of E. coli are postulated.
M W Pandit+2 more
openaire +2 more sources