Karyotype description and comparative chromosomal mapping of rDNA and U2 snDNA sequences in Eigenmannia limbata and E. microstoma (Teleostei, Gymnotiformes, Sternopygidae) [PDF]
Comparative Cytogenetics, 2022 The genus Eigenmannia Jordan et Evermann,1896 includes electric fishes endemic to the Neotropical region with extensive karyotype variability and occurrence of different sex chromosome systems, however, cytogenetic studies within this group are ...
Cristian Andrés Araya-Jaime +5 more
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Role of the particle's stepping cycle in an asymmetric exclusion process: A model of mRNA translation [PDF]
Phys. Rev. E 81, 051904 (2010), 2009 Messenger RNA translation is often studied by means of statistical-mechanical models based on the Asymmetric Simple Exclusion Process (ASEP), which considers hopping particles (the ribosomes) on a lattice (the polynucleotide chain). In this work we extend this class of models and consider the two fundamental steps of the ribosome's biochemical cycle ...
arxiv +1 more source
Frontiers in Physiology, 2019 Hawthorn spider mite, Amphitetranychus viennensis Zacher, is one of the most devastating pests of deciduous fruit trees. The overall goal of this research is to develop a standardized protocol for real-time quantitative reverse transcription PCR (RT-qPCR)
Jing Yang +9 more
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An RNA-binding complex involved in ribosome biogenesis contains a protein with homology to tRNA CCA-adding enzyme. [PDF]
PLoS Biology, 2013 A multitude of proteins and small nucleolar RNAs transiently associate with eukaryotic ribosomal RNAs to direct their modification and processing and the assembly of ribosomal proteins. Utp22 and Rrp7, two interacting proteins with no recognizable domain,
Jinzhong Lin +4 more
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Control of ribosome traffic by position-dependent choice of synonymous codons [PDF]
Phys. Biol. 10 (2013) 056011, 2013 Messenger RNA encodes a sequence of amino acids by using codons. For most amino acids there are multiple synonymous codons that can encode the amino acid. The translation speed can vary from one codon to another, thus there is room for changing the ribosome speed while keeping the amino acid sequence and hence the resulting protein.
arxiv +1 more source
Sandwich hybridisation assay for quantitative detection of yeast RNAs in crude cell lysates
Microbial Cell Factories, 2003 Background A rapid microtiter plate based sandwich hybridization assay was developed for detection and quantification of single RNA species using magnetic beads.
Molin Søren +5 more
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Distribution of dwell times of a ribosome: effects of infidelity, kinetic proofreading and ribosome crowding [PDF]
Physical Biology, 8 (2011) 026005, 2010 Ribosome is a molecular machine that polymerizes a protein where the sequence of the amino acid residues, the monomers of the protein, is dictated by the sequence of codons (triplets of nucleotides) on a messenger RNA (mRNA) that serves as the template. The ribosome is a molecular motor that utilizes the template mRNA strand also as the track. Thus, in
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Exploring valid reference genes for quantitative real-time PCR analysis in Sesamia inferens (Lepidoptera: Noctuidae). [PDF]
PLoS ONE, 2015 The pink stem borer, Sesamia inferens, which is endemic in China and other parts of Asia, is a major pest of rice and causes significant yield loss in this host plant. Very few studies have addressed gene expression in S. inferens. Quantitative real-time
Meng Sun +3 more
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Evaluation of three molecular markers for identification of European primary parasitoids of cereal aphids and their hyperparasitoids. [PDF]
PLoS ONE, 2017 Aphids are major pests of cereal crops and a suite of hymenopteran primary parasitoids play an important role in regulating their populations. However, hyperparasitoids may disrupt the biocontrol services provided by primary parasitoids.
Zhengpei Ye +3 more
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Analyzing ribosome remodeling in health and disease [PDF]
PROTEOMICS (2020), 2020 regulation largely unexplored, in part due to methodological limitations. Indeed, we review evidence demonstrating that commonly used methods, such as transcriptomics, are inadequate because the variability in mRNAs coding for ribosomal proteins (RP) does not necessarily correspond to RP variability.
arxiv +1 more source