Results 301 to 310 of about 122,279 (340)

Postmortem interval determination using 18S-rRNA and microRNA

Science & Justice, 2014
The importance of determining postmortem interval (PMI) is crucial to criminal, civil and forensic cases. The precise estimation of PMI is a critical step in many death investigations. A technique exploiting the level of RNA, 18S rRNA and microRNA to estimate PMI was investigated.
Wen-Can, Li   +8 more
openaire   +2 more sources

Reverse Transcription of 18S rRNA with Poly(dT)18 and Other Homopolymers

Plant Molecular Biology Reporter, 2012
Ribosomal 18S RNA is widely used as a housekeeping gene in expression studies, including end-point PCR, Northern analysis, and real-time experiments. However, there are two disadvantages and two points of error introduction in using 18S rRNA as a reference gene. First, 18S has no poly(A) tail, so it is commonly reverse transcribed with specific primers
Bogdanović, Milica   +7 more
openaire   +3 more sources

Details of Gastropod Phylogeny Inferred from 18S rRNA Sequences

Molecular Phylogenetics and Evolution, 1998
Some generally accepted viewpoints on the phylogenetic relationships within the molluscan class Gastropoda are reassessed by comparing complete 18S rRNA sequences. Phylogenetic analyses were performed using the neighbor-joining and maximum parsimony methods.
Winnepenninckx, Birgitta   +3 more
openaire   +3 more sources

Cryptomonad nuclear and nucleomorph 18S rRNA phylogeny

European Journal of Phycology, 1996
Nuclear and nucleomorph 18S ribosomal RNA genes from six cryptomonads were amplified by the polymerase chain reaction and sequenced. Phylogenetic trees were constructed by distance, parsimony, and maximum likelihood methods for all available cryptomonad nuclear and nucleomorph 18S rRNA sequences.
T. Cavalier-Smith   +6 more
openaire   +1 more source

18S rRNA sequences and phylogeny of platyhelminthes

Biochemical Systematics and Ecology, 1993
Abstract The phyletic relationships, and hence the systematic position, of freshwater planarians (Tricladida, Turbellaria, Platyhelminthes) within the Turbellaria, and of Platyhelminthes within the Metazoa are uncertain. This stems partially from the shortcomings of the characters and techniques clasically used in the systematic study of these groups.
Marta Riutort   +3 more
openaire   +1 more source

Performance of 18S rRNA in Littorinid Phylogeny (Gastropoda: Caenogastropoda)

Journal of Molecular Evolution, 1998
In the past, 18S rRNA sequences have proved to be very useful for tracing ancient divergences but were rarely used for resolving more recent ones. Moreover, it was suggested that the molecule does not contain useful information to resolve divergences which took place during less than 40 Myr.
B M, Winnepenninckx   +2 more
openaire   +2 more sources

Intermolecular hybridization of 5S rRNA with 18S rRNA: Identification of a 5′-terminally-located nucleotide sequence in mouse 5S rRNA which base-pairs with two specific complementary sequences in 18S rRNA

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1991
Eukaryotic 5S rRNA hybridizes specifically with 18S rRNA in vitro to form a stable intermolecular RNA:RNA hybrid. We have used 5S rRNA/18S rRNA fragment hybridization studies coupled with ribonuclease digestion and primer extension/chain termination analysis of 5S rRNA:18S rRNA hybrids to more completely map those mouse 5S rRNA and 18S rRNA sequences ...
K D, Sarge, E S, Maxwell
openaire   +2 more sources

18S rRNA sequences of Leishmania enriettii promastigote and amastigote

International Journal for Parasitology, 1991
Dideoxy sequencing with reverse transcriptase and universal primers was used to obtain partial sequences of the 18S rRNAs from the promastigote and amastigote life-cycle stages of L. enriettii. Approximately 1400 nucleotides of sequence from the two stages were compared.
K G, Field   +2 more
openaire   +2 more sources

Novelty in Phylogeny of Gastrotricha: Evidence from 18S rRNA Gene

Molecular Phylogenetics and Evolution, 1999
Gastrotricha form a phylum which is crucial for defining the origin of pseudocoelomates, in that they share a number of characters with Rotifera and Nematoda but also with acoelomates, and even the evolutionary relationships within the phylum are anything but defined.
WIRZ A   +4 more
openaire   +3 more sources

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