Validation of suitable internal control genes for expression studies in aging. [PDF]
Quantitative data from experiments of gene expression are often normalized through levels of housekeeping genes transcription by assuming that expression of these genes is highly uniform. This practice is being questioned as it becomes increasingly clear
Bacalini, MARIA GIULIA +9 more
core +1 more source
An RNA Conformational Switch Regulates Pre-18S rRNA Cleavage [PDF]
To produce mature ribosomal RNAs (rRNAs), polycistronic rRNA transcripts are cleaved in an ordered series of events. We have uncovered the molecular basis for the ordering of two essential cleavage steps at the 3'-end of 18S rRNA. Using in vitro and in vivo structure probing, RNA binding and cleavage experiments, and yeast genetics, we demonstrate that
Allison C, Lamanna, Katrin, Karbstein
openaire +2 more sources
Amplification efficiency and thermal stability of qPCR instrumentation: Current landscape and future perspectives [PDF]
This is a final draft version of the publication following acceptance by the journal.Quantitative polymerase chain reaction (qPCR) is a method of amplifying and detecting small samples of genetic material in real time and is in routine use across many ...
Karteris, E, Rogers-Broadway, KR
core +1 more source
Increased expression of aggrecan and biglycan mRNA in Achilles tendinopathy [PDF]
To determine the expression of mRNA encoding the proteoglycans aggrecan, versican, biglycan and decorin in mid-tendon samples of chronic painful Achilles tendinopathy and ruptured Achilles tendons, compared with normal tendons.
Corps, AN +5 more
core +1 more source
The 5′ end of the 18S rRNA can be positioned from within the mature rRNA [PDF]
In yeast, the 5' end of the mature 18S rRNA is generated by endonucleolytic cleavage at site A1, the position of which is specified by two distinct signals. An evolutionarily conserved sequence immediately upstream of the cleavage site has previously been shown to constitute one of these signals.
Sharma, K, Venema, J, Tollervey, D
openaire +3 more sources
Background The microbiome affects the health of plants and animals, including humans, and has many biological, ecological, and evolutionary consequences.
Kevin Xu Zhong +4 more
doaj +1 more source
An integrative description of a population of Mesobiotus radiatus (Pilato, Binda & Catanzaro, 1991) from Kenya [PDF]
In a moss sample collected from Mount Kulal in Kenya, a new population of Mesobiotus radiatus was found. Given that the original description of M. radiatus was based solely on the morphology observed by light microscopy and measurements based mostly on a
Kaczmarek, Łukasz +3 more
core +1 more source
Phylogeny of protostome worms derived from 18S rRNA sequences. [PDF]
The phylogenetic relationships of protostome worms were studied by comparing new complete 18S rRNA sequences of Vestimentifera, Pogonophora, Sipuncula, Echiura, Nemertea, and Annelida with existing 18S rRNA sequences of Mollusca, Arthropoda, Chordata, and Platyhelminthes.
Winnepenninckx, Birgitta +2 more
openaire +3 more sources
Identification of Sex and Female's Reproductive Stage in Commercial Fish Species through the Quantification of Ribosomal Transcripts in Gonads. [PDF]
The estimation of maturity and sex of fish stocks in European waters is a requirement of the EU Data Collection Framework as part of the policy to improve fisheries management.
Iratxe Rojo-Bartolomé +3 more
doaj +1 more source
Self-extracellular RNA (eRNA), which is released under pathological conditions from damaged tissue, has recently been identified as a new alarmin and synergistic agent together with toll-like receptor (TLR)2 ligands to induce proinflammatory activities ...
Karsten Grote +7 more
doaj +1 more source

