Results 31 to 40 of about 24,140 (251)

Collateral cleavage of 28s rRNA by RfxCas13d causes death of mice [PDF]

, 2022
Summary The CRISPR-Cas13 system is an RNA-guided RNA-targeting system, and has been widely used in transcriptome engineering with potentially important clinical applications. However, it is still controversial whether Cas13 exhibits collateral activity in mammalian cells. Here, we found that knocking down gene expression using RfxCas13d
Yunfei Li, Junjie Xu, Xuefei Guo, Zhiwei Li, Lili Cao, Shengde Liu, Ying Guo, Guodong Wang, Yujie Luo, Zeming Zhang, Xuemei Wei, Yingchi Zhao, Tongtong Liu, Xiao Wang, Huawei Xia, Ming Kuang, Qirui Guo, Junhong Li, Luoying Chen, Yibing Wang, Qi Li, Fengchao Wang, Qinghua Liu, Fuping You   +23 more
openaire   +1 more source

Identificación de Yarrowia lipolytica (Ascomycota: Hemiascomycetes) como contaminante en la obtención de amplificados del gen 28S rRNA de moluscos

Revista Peruana de Biología, 2011
En el presente trabajo se identifica una secuencia de DNA no esperada proveniente de los amplificados del gen 28S rRNA de moluscos terrestres. Las extracciones de DNA se realizaron del tejido del pie de caracoles terrestres por el método del CTAB ...
Jenny Chirinos, Carlos Congrains, Rina Ramírez, Pablo Ramírez   +3 more
doaj   +1 more source

DNA Sequencing Analysis of ITS and 28S rRNA of Poria cocos

Biological and Pharmaceutical Bulletin, 2007
We determined the DNA sequences of the internal transcribed spacer 1 and 2 (ITS 1 and 2), the 5.8S rRNA gene and most of the 28S rRNA gene of Poria cocos for the first time, and conducted analysis of 20 samples including cultured mycelias and crude drug materials obtained from various localities and markets. Direct sequencing of the ITS 1 and 2 regions
Atsumi, Toshiyuki, Kakiuchi, Nobuko, Mikage, Masayuki   +2 more
openaire   +3 more sources

Integrative Taxonomy and Molecular Phylogeny of the Plant-Parasitic Nematode Genus Paratylenchus (Nematoda: Paratylenchinae): Linking Species with Molecular Barcodes

Plants, 2021
Pin nematodes of the genus Paratylenchus are obligate ectoparasites of a wide variety of plants that are distributed worldwide. In this study, individual morphologically vouchered nematode specimens of fourteen Paratylenchus species, including P ...
Phougeishangbam Rolish Singh, Gerrit Karssen, Marjolein Couvreur, Sergei A. Subbotin, Wim Bert   +4 more
doaj   +1 more source

Genetic Diversity of Apis spp. in Thailand Inferred from 28S rRNA Nuclear and Cytochrome b Mitochondrial Gene Sequences

Psyche: A Journal of Entomology, 2019
Knowledge of the genetic diversity of Apis spp. is important in order to provide a better understanding of breeding strategies that relate to the conservation of wild species and colony survival of farmed species.
Thitipan Meemongkolkiat, Atsalek Rattanawannee, Chanpen Chanchao   +2 more
doaj   +1 more source

Phylogenetic analysis of phenotypically characterized Cryptococcus laurentii isolates reveals high frequency of cryptic species. [PDF]

PLoS ONE, 2014
Although Cryptococcus laurentii has been considered saprophytic and its taxonomy is still being described, several cases of human infections have already reported. This study aimed to evaluate molecular aspects of C.
Kennio Ferreira-Paim, Thatiana Bragine Ferreira, Leonardo Andrade-Silva, Delio Jose Mora, Deborah J Springer, Joseph Heitman, Fernanda Machado Fonseca, Dulcilena Matos, Márcia Souza Carvalho Melhem, Mario León Silva-Vergara   +9 more
doaj   +1 more source

From junk to function — How weak selection in eukaryotes builds new parts and drives genomic complexity

FEBS Letters, EarlyView.
How do genomes gain new functional parts? In eukaryotes, which tend to evolve under weak selection, much of the genome is junk. Palazzo and Qiu borrow the logic of Markov chains to show how non‐functional DNA becomes functional through the appearance of intermediate states, which arise due to epistasis, buffering, and biochemical messiness, allowing ...
Alexander F. Palazzo, Yi Qiu
wiley   +1 more source

Genetic features of the marine polychaete Sirsoe methanicola from metagenomic data

Frontiers in Marine Science, 2023
The methane ice worm Sirsoe methanicola is the only marine polychaete species observed to colonize the methane hydrates of the Gulf of Mexico. Methane hydrates are ephemeral features of deep-sea cold seeps, and finding worm-colonized hydrates is rare ...
Shen Jean Lim, Luke R. Thompson, Luke R. Thompson, Kelly D. Goodwin   +3 more
doaj   +1 more source

A context‐dependent modulatory role for eIF6 in acquired resistance to vemurafenib in melanoma

FEBS Letters, EarlyView.
Acquired resistance to vemurafenib upregulates the translation factor eIF6 in melanoma cells. Silencing eIF6 in resistant cells reduces proliferation and partially restores drug sensitivity, whereas its overexpression increases sensitivity across melanoma lines regardless of BRAF status, via modulation of mTOR, S6K, and MAPK signaling.
George Kyriakopoulos, Antonia Petropoulou, Alexandra Anastogianni, Angelina Bania, Vassilis Stamatakis, Argyris Alexiou, Chi‐Ping Day, Glenn Merlino, Katerina Grafanaki, Constantinos Stathopoulos   +9 more
wiley   +1 more source

Alteration of rRNA gene copy number and expression in patients with intellectual disability and heteromorphic acrocentric chromosomes

Egyptian Journal of Medical Human Genetics, 2018
Background: Intellectual disability (ID) is an important medical and social problem that can be caused by different genetic and environmental factors. One such factor could be rDNA amplification and changes in rRNA expression and maturation.
Irina S. Kolesnikova, Alexander A. Dolskiy, Natalya A. Lemskaya, Yulia V. Maksimova, Asia R. Shorina, Alexander S. Graphodatsky, Ekaterina M. Galanina, Dmitry V. Yudkin   +7 more
doaj   +1 more source