Results 151 to 160 of about 3,824,869 (307)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Cette visualisation en 3D de poumons endommagés par Covid-19 permet de mieux comprendre le danger

, 2020
" Une vidéo en 3D montre les dommages aux poumons d'une personne atteinte du Covid-19. Diffusée par le Dr. Keith Mortman, qui travaille à l'hôpital universitaire de George-Washington, aux Etats-Unis, la vidéo a été produite en partenariat avec Surgical ...
Consortium 3D SHS Team
core  

3D Imaging and Measurement of Optic Nerve Sheath Diameter. [PDF]

Transl Vis Sci Technol
Silverman RH, Venuat C, Letoffet L, Ketterling JA.   +3 more
europepmc   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

3D Imaging of the Comparative Anatomical Structure of the Ossa Cranii in Some Ruminants. [PDF]

Vet Sci
Baygeldi SB, Aslan Kanmaz Y, Yurttaş H.
europepmc   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Cryo-FIB-enabled 3D imaging of biological specimens. [PDF]

Appl Microsc
Son S, Lee S, Kim J, Song C.
europepmc   +1 more source

Salmonella enterica serovar typhi limits the potency of typhoid toxin and ADP‐ribosylating toxin AB to establish a persistent infection

FEBS Open Bio, EarlyView.
The two catalytic subunits of typhoid toxin dissociate from the holotoxin in the ER of an intoxicated cell, but only CdtB exits the ER to generate immunosuppressive effects. PltA is retained in the ER and sequestered from its cytosolic target, thus allowing the anti‐inflammatory effects of CdtB to promote intestinal colonization.
Maria C. Zabala‐Rodriguez, Juliette K. Tinker, Teresa Frisan, Taj Azarian, Ken Teter   +4 more
wiley   +1 more source

Exploring the Feasibility of an Insourced 3D-Imaging Reconstruction: Preliminary Series with the Use of Synapse3D™ Software. [PDF]

Cancers (Basel)
Fettucciari D, Bracco M, Carerj C, Gavi F, Rossi F, Pallotta G, Assumma S, Panio E, Francocci A, Cavarra V, Montesi M, Testori N, Russo P, Turri FM, Totaro A, Gandi C, Foschi N, D'Amico L, Bizzarri FP, Sacco E, Pavone M, Gaia G, Rocco BMC, Sighinolfi MC.   +23 more
europepmc   +1 more source