Three-dimensional virtual refocusing of fluorescence microscopy images using deep learning
, 2019 Three-dimensional (3D) fluorescence microscopy in general requires axial scanning to capture images of a sample at different planes. Here we demonstrate that a deep convolutional neural network can be trained to virtually refocus a 2D fluorescence image ...
Ben-David, Eyal +7 more
core +1 more source
Voxel-wise comparisons of cellular microstructure and diffusion-MRI in mouse hippocampus using 3D Bridging of Optically-clear histology with Neuroimaging Data (3D-BOND) [PDF]
, 2018 A key challenge in medical imaging is determining a precise correspondence between image properties and tissue microstructure. This comparison is hindered by disparate scales and resolutions between medical imaging and histology.
Ball, G +6 more
core +6 more sources
Terahertz Spectroscopic Signatures of Microcystin Aptamer Solution Probed with a Microfluidic Chip
Sensors, 2019 Terahertz signature detection of biological samples in aqueous solution remains a great challenge due to the strong terahertz absorption of water. Here we propose a new preparation process for fabricating a microfluidic chip and use it as an effective ...
Mingkun Zhang +7 more
doaj +1 more source
Deformation Detection Method of Mine Tunnel Based on Mobile Detection System
Sensors, 2020 Subway structure safety detection is an important method to ensure the safe operation of trains. Efficient, high-precision, and automatic tunnel clearance detection is the key to ensure safe operations.
Jian Sun +3 more
doaj +1 more source
3D Imaging of a Phase Object from a Single Sample Orientation Using an Optical Laser
, 2011 Ankylography is a new 3D imaging technique, which, under certain circumstances, enables reconstruction of a 3D object from a single sample orientation. Here, we provide a matrix rank analysis to explain the principle of ankylography.
Chen, Chien-Chun +6 more
core +1 more source
Revealing the structure of land plant photosystem II: the journey from negative‐stain EM to cryo‐EM
FEBS Letters, EarlyView.Advances in cryo‐EM have revealed the detailed structure of Photosystem II, a key protein complex driving photosynthesis. This review traces the journey from early low‐resolution images to high‐resolution models, highlighting how these discoveries deepen our understanding of light harvesting and energy conversion in plants.
Roman Kouřil
wiley +1 more source
Deep learning enhanced NIR-II volumetric imaging of whole mice vasculature
Opto-Electronic Advances, 2023 Fluorescence imaging through the second near-infrared window (NIR-II,1000–1700 nm) allows in-depth imaging. However, current imaging systems use wide-field illumination and can only provide low-contrast 2D information, without depth resolution.
Sitong Wu +7 more
doaj +1 more source
FEBS Letters, EarlyView.Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi +5 more
wiley +1 more source
3D imaging lipidometry in single cell by in-flow holographic tomography
Opto-Electronic Advances, 2023 The most recent discoveries in the biochemical field are highlighting the increasingly important role of lipid droplets (LDs) in several regulatory mechanisms in living cells.
Daniele Pirone +12 more
doaj +1 more source
Organoids in pediatric cancer research
FEBS Letters, EarlyView.Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley +1 more source