We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Text-to-hierarchical three-dimensional scene generation: a new approach for layered three-dimensional modeling from natural language. [PDF]
Gu Z, Gao T, Zhao L, Liu H.
europepmc +1 more source
Heterotropic regulation and negative homotropic cooperativity
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
A novel technique for automatic hybrid modulation classification in UWA communications using 3D constellation diagrams. [PDF]
Abdel-Moneim MA +4 more
europepmc +1 more source
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source
Evaluating the Incremental Value of Three-Dimensional Conformer Descriptors for Blood-Brain Barrier Permeability Prediction Using the B3DB Benchmark Dataset. [PDF]
Tiwari S +3 more
europepmc +1 more source
GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain +11 more
wiley +1 more source
Mamba-BEV: A Multiscale State-Space Framework for 3D Object Detection from Point Clouds. [PDF]
Liu Y +6 more
europepmc +1 more source
Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer +7 more
wiley +1 more source
Open Long-Tailed Multimodal 3D Model Classification Based on Sample-Enhanced Category-Space Learning. [PDF]
Wang Y, Gao X, Zhang C, Xue Y.
europepmc +1 more source

