Results 51 to 60 of about 272,217 (266)

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

An In-Depth Analysis of Peritoneal Dialysate Effluent Composition with a Deep-UV-LED-Based Affordable Optical Chromatographic Sensor

Engineering Proceedings
It was shown earlier that the use of fast protein and metabolites liquid chromatography (FPMLC) and low-cost deep UV–LED-based optical chromatographic sensors with PD-10 desalting columns as a separation element can facilitate the monitoring of patients ...
Nikolay Ovsyannikov, Georgii Konoplev, Artur Kuznetsov, Alar Sünter, Vadim Korsakov, Oksana Stepanova, Milana Mikhailis, Roman Gerasimchuk, Alina Isachkina, Zarina Rustamova, Aleksandr Frorip   +10 more
doaj   +1 more source

Enzymatic degradation of biopolymers in amorphous and molten states: mechanisms and applications

FEBS Open Bio, EarlyView.
This review explains how polymer morphology and thermal state shape enzymatic degradation pathways, comparing amorphous and molten biopolymer structures. By integrating structure–reactivity principles with insights from thermodynamics and enzyme engineering, it highlights mechanisms that enable efficient polymer breakdown.
Anđela Pustak, Aleksandra Maršavelski
wiley   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

Anion Binding Studies on Receptors Derived from the Indolo[2,3-a]carbazole Scaffold Having Different Binding Cavity Sizes

Sensors, 2014
The indolo[2,3-a]carbazole scaffold is a fused polyheteroaromatic system bearing two NH groups which suitably converge as hydrogen bond donor sites for the recognition of anions. A simple derivatisation of the indolocarbazole system at positions 1 and 10
Guzmán Sánchez, David Curiel, Alberto Tárraga, Pedro Molina   +3 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Germanium-based integrated photonics from near- to mid-infrared applications

Nanophotonics, 2018
Germanium (Ge) has played a key role in silicon photonics as an enabling material for datacom applications. Indeed, the unique properties of Ge have been leveraged to develop high performance integrated photodectors, which are now mature devices.
Marris-Morini Delphine, Vakarin Vladyslav, Ramirez Joan Manel, Liu Qiankun, Ballabio Andrea, Frigerio Jacopo, Montesinos Miguel, Alonso-Ramos Carlos, Le Roux Xavier, Serna Samuel, Benedikovic Daniel, Chrastina Daniel, Vivien Laurent, Isella Giovanni   +13 more
doaj   +1 more source

Fluoride Anion Recognition by a Multifunctional Urea Derivative: An Experimental and Theoretical Study

Sensors, 2016
In this work we demonstrate the ability of a multifaceted N,N′-disubstituted urea to selectively recognize fluoride anion (F−) among other halides. This additional function is now added to its already reported organocatalytic and organogelator properties.
Jana Schiller, Raúl Pérez-Ruiz, Diego Sampedro, Eugenia Marqués-López, Raquel P. Herrera, David Díaz Díaz   +5 more
doaj   +1 more source

X-Ray Absorption Spectroscopy

Acta Physica Polonica A, 1994
X-ray absorption spectroscopy, today widely used as a tool for structural investigations, is described. Theoretically it is shown that above an absorption edge the absorption coefficient is well approximated by a sum of terms, each one describing a particular path followed by the photoelectron outgoing from the atom.
openaire   +2 more sources