Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Socioeconomic burden of osteoarthritis in Sub-Saharan Africa and the determinants of healthcare inequities: a scoping review protocol. [PDF]
Andela L +4 more
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Letter from the Alachua County Abstract Company to William P. Clyde
Letter from the Alachua County Abstract Company to William P. Clyde.
Alachua County Abstract Company
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Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao +3 more
wiley +1 more source
Correction: Reconstruction algorithms and arm positioning effects on abdominal CT image quality and radiation dose: a phantom study. [PDF]
Mun HS, Lim S, Kang S.
europepmc +1 more source
POSHAN’s abstract digest on maternal and child nutrition research - Issue 44
Issue 44 of the POSHAN Abstract Digest, brings to you yet another collection of articles on various topics pertaining to maternal and child nutrition.
Avula, Rasmi
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Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Effect of Vitamin D Supplementation on Total Knee Arthroplasty Outcomes: A Systematic Review. [PDF]
Vosoughi F +3 more
europepmc +1 more source
Letter from Alachua County Abstract Company to Travellers [sic] Insurance Company
Letter from the president of the Alachua County Abstract Company to the Travellers [sic] Insurance Company. The correspondence is dated 5 September 1912 and outlines possible loan terms for the financing of Dixieland College in Gainesville ...
Alachua County Abstract Company
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