Results 141 to 150 of about 4,001 (176)

Identification and characterization of an acetyl xylan esterase from Aspergillus oryzae

Journal of Bioscience and Bioengineering, 2021
In this study, we report the identification and characterization of an acetyl xylan esterase, designated as AoAXEC, which was previously annotated as a hypothetical protein encoded by AO090023000158 in the Aspergillus oryzae genomic database. Based on its amino acid sequence, a low sequence identity to known acetyl xylan esterases was observed in the ...
Tomoe Kato, Yoshihito Shiono, Takuya Koseki   +2 more
openaire   +4 more sources

Distinct roles of carbohydrate esterase family CE16 acetyl esterases and polymer-acting acetyl xylan esterases in xylan deacetylation

Journal of Biotechnology, 2013
Mass spectrometric analysis was used to compare the roles of two acetyl esterases (AE, carbohydrate esterase family CE16) and three acetyl xylan esterases (AXE, families CE1 and CE5) in deacetylation of natural substrates, neutral (linear) and 4-O-methyl glucuronic acid (MeGlcA) substituted xylooligosaccharides (XOS).
S. Koutaniemi, M.P. van Gool, M. Juvonen, J. Jokela, S.W. Hinz, H.A. Schols, M. Tenkanen   +6 more
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Acetyl xylan esterase-catalyzed deacetylation of chitin and chitosan

Carbohydrate Polymers, 2006
Acetyl xylan esterase catalyzes the hydrolysis of N-acetyl groups in chitinous materials of variable degrees of polymerization and acetylation. The influence of substrate accessibility is most notable with substrate of high degree of acetylation (DA). The activity rises sharply as the number of acetyl groups in the substrates decreases and at about 24%
K MORLEY, G CHAUVE, R KAZLAUSKAS, C DUPONT, F SHARECK, R MARCHESSAULT   +5 more
openaire   +3 more sources

Cooperativity of Esterases and Xylanases in the Enzymatic Degradation of Acetyl Xylan

Nature Biotechnology, 1986
Xylan exists in many plants in an acetylated form, a circumstance largely neglected in studies of its breakdown by microbial enzymes. The present investigation demonstrates that fungal esterase and xylanase activities act cooperatively in hydrolyzing acetyl xylan.
Biely, P., MacKenzie, C. R., Puls, J., Schneider, H.   +3 more
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Purification and characterization of an acetyl xylan esterase from Aspergillus niger

Journal of Biotechnology, 1993
Abstract An acetyl esterase has been purified from an Aspergillus niger culture filtrate based on its activity towards p -nitrophenyl acetate. Its molecular weight determined by SDS-PAGE, was 30480; its iso-electric point was around 3.0–3.2. When tested on steamed birchwood xylan, acetylated apple or sugar beet pectin, only in the case of steamed ...
Kormelink, F.J.M., Lefebvre, B., Strozyk, F., Voragen, A.G.J.   +3 more
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Trichoderma longibrachiatum acetyl xylan esterase 1 enhances hemicellulolytic preparations to degrade corn silage polysaccharides

Bioresource Technology, 2014
Supplementation of a Trichoderma longibrachiatum preparation to an industrial Aspergillus niger/Talaromyces emersonii enzyme mixture demonstrated synergy for the saccharification of corn silage water-unextractable solids (WUS). Sub-fractions of the crude T.
Neumüller, K.G., Streekstra, H., Gruppen, H., Schols, H.A.   +3 more
openaire   +4 more sources

Products of hydrolysis of beechwood acetyl-4-O-methylglucuronoxylan by a xylanase and an acetyl xylan esterase

Enzyme and Microbial Technology, 1991
Simultaneous hydrolysis of acetyl-4-O-methylglucuronoxylan by xylanase and acetyl xylan esterase leads to the highest recoveries of acetic acid, xylobrise, and xylotriese. Treatment with xylanase prior to deacetylation leads to a broad spectrum of neutral and uronic acid-substituted xylooligomers, whereas deacetylation followed by xylanase hydrolysis ...
Puls, Jurgen, Tenkanen, Maija, Korte, Hans, Poutanen, Kaisa   +3 more
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Colorimetric Detection of Acetyl Xylan Esterase Activities

2017
Colorimetric detection of reaction products is typically preferred for initial surveys of acetyl xylan esterase (AcXE) activity. This chapter will describe common colorimetric methods, and variations thereof, for measuring AcXE activities on commercial, synthesized, and natural substrates.
Mai-Gisondi, Galina, Master, Emma R.
openaire   +4 more sources

Characterization and mode of action of two acetyl xylan esterases from Chrysosporium lucknowense C1 active towards acetylated xylans

Enzyme and Microbial Technology, 2011
Two novel acetyl xylan esterases, Axe2 and Axe3, from Chrysosporium lucknowense (C1), belonging to the carbohydrate esterase families 5 and 1, respectively, were purified and biochemically characterized. Axe2 and Axe3 are able to hydrolyze acetyl groups both from simple acetylated xylo-oligosaccharides and complex non-soluble acetylglucuronoxylan. Both
Pouvreau, L.A.M., Jonathan, M.C., Kabel, M.A., Hinz, S.W.A., Gruppen, H., Schols, H.A.   +5 more
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Deacetylation of xylans by acetyl esterases of Trichoderma reesei

Applied Microbiology and Biotechnology, 1990
Two previously purified esterases of Trichoderma reesei were used to study the deacetylation of polymeric, oligomeric and dimeric acetylated xylan fragments. For the first time nearly complete enzymatic deacetylation of polymeric xylan with purified acetyl xylan esterase was demonstrated, resulting in precipitation of the remaining polymer structure ...
Sundberg, Maija, Korte, Hans, Puls, Jurgen, Poutanen, Kaisa   +4 more
openaire   +1 more source