Results 151 to 160 of about 4,001 (176)

Purification and characterization of two acetyl xylan esterases from Penicillium purpurogenum

Biotechnology and Applied Biochemistry, 1996
Penicillium purpurogenum produces several enzymes active in xylan hydrolysis, of there, the acetyl xylan esterase (AXE) activity secreted by the fungus has now been studied. The amount of activity obtained in the culture is related to the degree of acetylation of the carbon source used, the best being chemically acetylated xylan.
L, Egaña, R, Gutiérrez, V, Caputo, A, Peirano, J, Steiner, J, Eyzaguirre   +5 more
openaire   +2 more sources

Comparison of Catalytic Properties of Acetyl Xylan Esterases from Three Carbohydrate Esterase Families

2003
Most natural hemicelluloses are acetylated. Some xylans carryalso esterified phenolic acid side groups. Carbohydrate-actingesterases (CE) have been recently classified according tostructural similarities into several CE families. However, thisclassification does not necessarily predict the substrate specificity of these enzymes.
Tenkanen, Maija, Eyzaguirre, Jaime, Isoniemi, Riitta, Faulds, Craig B., Biely, Peter   +4 more
openaire   +4 more sources

A colorimetric assay for the determination of acetyl xylan esterase or cephalosporin C acetyl esterase activities using 7-amino cephalosporanic acid, cephalosporin C, or acetylated xylan as substrate

Analytical Biochemistry, 2007
A bromothymol blue-based colorimetric assay has been devised to screen for acetyl xylan esterase or cephalosporin C (CPC) deacetylase activities using 7-amino cephalosporanic acid (7-ACA), CPC, or acetylated xylan as substrate. These enzymes are not screened with their natural substrates because of the tedious procedures available previously.
Irene, Martínez-Martínez, Silvia, Montoro-García, José Daniel, Lozada-Ramírez, Alvaro, Sánchez-Ferrer, Francisco, García-Carmona   +4 more
openaire   +2 more sources

Hydrolysis of Wheat Arabinoxylan by Two Acetyl Xylan Esterases from Chaetomium thermophilum

Applied Biochemistry and Biotechnology, 2014
The thermophilic filamentous ascomycete Chaetomium thermophilum produces functionally diverse hemicellulases when grown on hemicellulose as carbon source. Acetyl xylan esterase (EC 3.1.1.72) is an important accessory enzyme in hemicellulose biodegradation. Although the genome of C.
Tong, Xiaoxue, Lange, Lene, Grell, Morten Nedergaard, Busk, Peter Kamp   +3 more
openaire   +3 more sources

A unique CE16 acetyl esterase from Podospora anserina active on polymeric xylan

Applied Microbiology and Biotechnology, 2015
The genome of the coprophilous fungus Podospora anserina displays an impressive array of genes encoding hemicellulolytic enzymes. In this study, we focused on a putative carbohydrate esterase (CE) from family 16 (CE16) that bears a carbohydrate-binding module from family CBM1.
Puchart, Vladimir, Berrin, Jean-Guy, Haon, Mireille, Biely, Peter   +3 more
openaire   +4 more sources

Regioselective deacetylation of cellulose acetates by acetyl xylan esterases of different CE-families

Journal of Biotechnology, 2003
Cellulose acetate (CA) was found to be a substrate of several acetyl xylan esterases (AXE). Eight AXE from different carbohydrate esterase (CE) families were tested on their activity against CA with a degree of substitution of 0.7 and 1.4. The classification of the AXEs into CE families according to their structure by hydrophobic cluster analysis ...
Altaner, Clemens, Saake, Bodo, Tenkanen, Maija, Eyzaguirre, Jaime, Faulds, Craig B., Biely, Peter, Viikari, Liisa, Siika-aho, Matti, Puls, Jürgen   +8 more
openaire   +4 more sources

Purification and Characterization of an Acetyl Xylan Esterase fromBacillus pumilus

Applied and Environmental Microbiology, 1998
ABSTRACTBacillus pumilusPS213 was found to be able to release acetate from acetylated xylan. The enzyme catalyzing this reaction has been purified to homogeneity and characterized. The enzyme was secreted, and its production was induced by corncob powder and xylan.
G, Degrassi, B C, Okeke, C V, Bruschi, V, Venturi   +3 more
openaire   +2 more sources

Heterologous expression and biochemical characterization of acetyl xylan esterase from Coprinopsis cinerea

World Journal of Microbiology and Biotechnology, 2012
Acetyl xylan esterase (AXE) from basidiomycete Coprinopsis cinerea Okayama 7 (#130) was functionally expressed in Pichia pastoris with a C-terminal tag under the alcohol oxidase 1 (AOX1) promoter and secreted into the medium at 1.5 mg l(-1). Its molecular mass was estimated to be 65.5 kDa based on the SDS-PAGE analysis, which is higher than the ...
Veeresh, Juturu, Christina, Aust, Jin Chuan, Wu   +2 more
openaire   +2 more sources

Biochemical characterization of an esterase from Thermobifida fusca YX with acetyl xylan esterase activity

Molecular Biology Reports
Esterases (EC 3.1.1.X) are enzymes that catalyze the hydrolysis ester bonds. These enzymes have large potential for diverse applications in fine industries, particularly in pharmaceuticals, cosmetics, and bioethanol production.In this study, a gene encoding an esterase from Thermobifida fusca YX (TfEst) was successfully cloned, and its product was ...
Adriana S, da Silva, Patricia P, Adriani, Gabriel S, de Oliveira, Adriana Rios Lopes, Rocha, Elen A, Perpétuo, Marcio V B, Dias, Felipe S, Chambergo   +6 more
openaire   +2 more sources

Production of acetyl xylan esterase by Trichoderma reesei and Schizophyllum commune

Canadian Journal of Microbiology, 1988
Activity of acetyl xylan esterase, an enzyme that removes acetyl groups from acetyl xylan, was coproduced with that of endoxylanase and endoglucanase in two Trichoderma reesei strains and one Schizophyllum commune strain. The levels of activity of extracellular enzymes were measured during the course of cultivation on different carbon sources.
Biely, P., MacKenzie, Colin, Schneider, H.   +2 more
openaire   +2 more sources