Results 181 to 190 of about 1,481,033 (218)

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Guava Byproducts (<i>Psidium guajava</i> L.) as a Source of Phenolic Compounds with In Vitro Antihyperlipidemic Potential. [PDF]

Molecules
Baeza-Jiménez R, Noriega-Rodríguez JA, Villegas-Ochoa MA, Gonzalez-Aguilar GA, López-Martínez LX.   +4 more
europepmc   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Identification of alpha1-oleate as a potent regulator of adipokine-dependent metabolism, in bladder cancer tissue. [PDF]

Cancer Metab
Haq F, Chinchankar S, Ambite I, Ahmadi S, Tran H, Nazari A, Brisuda A, Hacek J, Babjuk M, Svanborg C.   +9 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Uncovering Bioactive Compounds in Propolis Extracts Prior to Isolation Through NMR Chemometric Analysis. [PDF]

Molecules
Stavropoulou MI, Cheilari A, Graikou K, Chinou I, Aligiannis N.   +4 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Phytochemical Enrichment of Carrot Seed Extracts by Ethanol-Modified Supercritical Fluid Extraction: Antimicrobial, Enzyme-Inhibitory, Butyrylcholinesterase Inhibition and Molecular Docking Investigations. [PDF]

Foods
Qanash H, Alsalamah SA, Bazaid AS, Almarshadi F, Alghonaim MI, Hakami W, Duhduh A, Almimoni NM.   +7 more
europepmc   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Effectiveness of Natural Deep Eutectic Solvents (NADES) in Extracting Bioactive Compounds from <i>Salvia sclarea</i>: HPLC-DAD Analysis, Antioxidant and Enzymes Inhibition Potential Evaluation. [PDF]

ACS Omega
Quradha MM, Duru ME, Kucukaydin S, Qahtan AMF, Khan R, Al Hashedi SA.   +5 more
europepmc   +1 more source