Results 201 to 210 of about 2,365,735 (268)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Acquired resistance to the RAS(ON) multi-selective inhibitor daraxonrasib guides rational combination therapy strategies in pancreatic cancer. [PDF]

Nat Med
Aronchik I, Kar S, Zhuang Y, Ahler E, Lai LP, Seshadri V, Yang YC, Bulle A, Menard M, Nayak B, Labrecque MP, Dilly J, Kim E, Jiang L, Yano J, Wasko UN, Helland C, Bredeson S, Garrick B, Gindin Y, Sickler B, Wei X, Seamon K, Jiang J, Lim KH, Holderfield M, Quintana E, Hegde A, Salman Z, Starodub A, Spira A, Park W, Hong DS, Barve M, Pelster M, Sommerhalder D, Punekar SR, Garrido-Laguna I, Wolpin BM, Maitra A, Gustafson WC, Kelsey S, Smith JAM, Lin KK, Aguirre AJ, Singh M.   +45 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

From immune checkpoint blockade to metabolic ecosystem intervention: A new perspective on ammonia-mediated remodelling of tumour immunity. [PDF]

Clin Transl Med
Li Y, Zhu Y, Gu J.
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Acquired Hemophilia A Revealing Occult Splenic Marginal Zone Lymphoma. [PDF]

Am J Case Rep
Brahmanapalle HS, Arora A.
europepmc   +1 more source

Navigator readouts with optimized processing improve spinal cord and gray matter segmentability in multi-echo gradient-echo imaging. [PDF]

Imaging Neurosci (Camb)
Büeler S, Beghini L, Kündig CW, Liechti MD, Vannesjo SJ, David G.   +5 more
europepmc   +1 more source

Evaluation of in vitro activity of delafloxacin against Staphylococcus aureus MSSA and MRSA isolated from a Brazilian tertiary hospital. [PDF]

Braz J Microbiol
Barth PO, Ucha LR, Lutz L, Barth AL.
europepmc   +1 more source

AI performance varies considerably across mammography devices: a multi-site and multi-vendor retrospective study. [PDF]

Insights Imaging
Blum M, Morant R, Eichenberger A, Geissler A, Subelack J, Vogel J, Ehlig D.   +6 more
europepmc   +1 more source