Results 181 to 190 of about 1,137,474 (237)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

open access: yesFEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

open access: yesFEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour   +2 more
wiley   +1 more source
Some of the next articles are maybe not open access.

The Acquisition of Syntax

Monographs of the Society for Research in Child Development, 1964
them to go beyond the speech they have actually heard and practiced to the creation of lawful novelties. If new monosyllables are created, speakers of English will agree that stug is "better English" than ftug. Probably this is because they have a shared implicit knowledge of the initial consonant clusters that are acceptable in English.
R, BROWN, C, FRASER
openaire   +2 more sources

Acquisition for Sleep

SSRN Electronic Journal, 2020
Abstract Within the policy debate, there is a fear that large incumbent firms buy small firms’ inventions to ensure that they are not used in the market. We show that such “acquisitions for sleep” can occur if and only if the quality of a process invention is small; otherwise, the entry profit will be higher than the entry-deterring ...
Norbäck, Pehr-Johan   +2 more
openaire   +2 more sources

Integrating knowledge acquisition and language acquisition

Applied Intelligence, 1992
Very large knowledge bases (KB's) constitute an important step for artificial intelligence and will have significant effects on the field of natural language processing. This thesis addresses the problem of effectively acquiring two large bodies of formalized knowledge: knowledge about the world (a KB), and knowledge about words (a lexicon).
openaire   +1 more source

The acquisition of semantics

WIREs Cognitive Science, 2010
AbstractThis review piece looks at how children acquire various elements of linguistic meaning. It considers issues in the acquisition of word meaning, argument structure, tense and aspect, and quantification and scope. For each of these areas, it considers the problems they pose for the acquisition process in general, reviews basic findings from the ...
openaire   +2 more sources

The Acquisition of an Appetite

Psychological Science, 2000
Unlike older animals, weanling-age rats do not seek water to drink when they are dehydrated, despite the fact that a physiological sensitivity to dehydration is present very soon after birth. We demonstrate here that the appetitive behaviors needed to approach and obtain water become linked to dehydration only as a result of specific postnatal ...
W G, Hall, H M, Arnold, K P, Myers
openaire   +2 more sources

PHOSPHATE ACQUISITION

Annual Review of Plant Physiology and Plant Molecular Biology, 1999
▪ Abstract  Phosphorus is one of the major plant nutrients that is least available in the soil. Consequently, plants have developed numerous morphological, physiological, biochemical, and molecular adaptations to acquire phosphate (Pi). Enhanced ability to acquire Pi and altered gene expression are the hallmarks of plant adaptation to Pi deficiency ...
openaire   +2 more sources

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