Results 131 to 140 of about 3,307,588 (255)

Repair of single-strand breaks during the cell cycle - saturation of repair capacity

Nucleus
Imaging of recruitment of XRCC1 and PCNA to a predetermined number of DNA single-strand breaks (SSB) demonstrates that (i) short-patch base excision repair is active throughout the whole cell cycle and repairs SSBs in over 60% of nonreplicating cells ...
Oskar Szelest, Agnieszka Hoang-Bujnowicz, Jurek W. Dobrucki, Mirosław Zarębski   +3 more
doaj   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Trend Breaks and Seasonality in the Yugoslav Black Market for Dollars, 1974-1987 [PDF]

We estimate a model of the black market premium for dollars in Yugoslavia from 1974-1987. Unlike previous applications of the model, our analysis addresses nonstationarity in the underlying data by allowing for trend breaks.
Steven Millsaps, John Dawson, Mark Strazicich   +2 more
core  

Active breaks as a physical education strategy to develop attention, concentration and attentional variability in higher education

BMC Psychology
Background Active breaks are an effective tool for introducing physical activity into the classroom and improving students’ physical and mental health. This study aims (a) to evaluate changes in attention, concentration and attentional stability levels ...
José Luis Ubago-Jiménez, Gabriel González-Valero, Félix Zurita-Ortega, Eduardo Melguizo-Ibáñez, José Manuel Alonso-Vargas   +4 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Teachers’ perceptions of the implementation of active breaks in the classroom: Is being active so important? [PDF]

Active breaks can be a reference framework for promoting physical activity at schools, hence the important of ascertaining which variables can promote or hinder their implementation.
Ponseti Verdaguer, Francisco Javier, Cantallops Ramón, Jaume, Sánchez Romero, Elisa Isabel, Muntaner Mas, Adriá   +3 more
core   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

Structural Breaks in the Real Exchange Rate and Real Interest Rate Relationship [PDF]

In this paper we empirically examine the relationship between the real exchange rate and real interest rate differentials using recent econometric methods robust to potential structural breaks.
Jun Nagayasu, Joseph P. Byrne
core