Results 61 to 70 of about 26,758 (195)
On the non-actuality inferences in Turkish avertives
Dilbilim Araştırmaları DergisiLanguages have various forms of expressing implications ofimminency and non-actuality. While a variety of constructions such asimperfectives, proximatives or adverbs like almost crosslinguistically imply thecounterfactuality of the event in question ...
Furkan DİKMEN, Ömer DEMİROK
doaj +1 more source
From Actuality to Concept in Hegel’s Logic
, 2017 This chapter examines Hegel’s treatment of the concept of actuality in his Science of Logic. It argues that Hegel’s treatment of actuality serves two functions: first, it provides the argument for the ‘genesis of the Concept’, Hegel’s version of Kant’s ...
Karen Ng
core +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley +1 more source
The Logic of Contingent Actuality
Ergo, An Open Access Journal of PhilosophyCurrent orthodoxy in modal logic and metaphysics has it that actuality is non-contingent in the following sense: for all p, if actually, p, then necessarily, actually, p. Call this thesis (Actuality) Necessitism and its negation (Actuality) Contingentism.
Martin Glazier, Stephan Krämer
doaj +2 more sources
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
FEBS Open Bio, EarlyView.BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Danica Andrejević, the interpreter of the recent Kosovo-Metohian literature [PDF]
Baština, 2013 One of the rare interpreter and preserver of the modern literary creativity of the Serbs in Kosovo and Metohia, Danica Andrejević, via her texts, monograph publications, lexicons and anthologies, has been systematizing and situating this literature for ...
Denić Sunčica
doaj
FEBS Open Bio, EarlyView.Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta +7 more
wiley +1 more source
Aristotle and Heidegger: Potentiality in Excess of Actuality
, 2016 Aristotle claims in book 9 of the Metaphysics that potentiality is distinct from actuality yet also that potentiality exists only for the sake of actuality. This essay presents the relationship between potentiality’s existence and actuality’s priority as
Brown, Nahum Carl HUMA
core +1 more source