Results 51 to 60 of about 39,121 (295)
Comparing the paths of Hedgehog release in artificial and natural settings
FEBS Letters, EarlyView.Hh morphogens require solubilization factors to overcome their tight association with the plasma membrane and signal to distant cells, but the precise functions of these factors are controversial. Here, we use published in vivo observations as the most relevant evidence to test their suggested functions and discuss contradictions and gaps between in ...
Janna Puschmann, Kay Grobe
wiley +1 more source
Molecular Oncology, EarlyView.Matched spatial transcriptomics and single‐nuclei RNA‐seq were generated for anaplastic and BRAFV600E papillary thyroid cancers revealing generic and tumor‐specific states occurring in cancer cells and in the tumor microenvironment. In this context, cancer dedifferentiation mirrored organoid maturation through ordered thyroid marker gain/loss ...
Adrien Tourneur +11 more
wiley +1 more source
To Repeat or Not To Repeat: That is the Question
Performance Philosophy, 2017 I had never been in Copenhagen before, yet this was my second visit to the city. The purpose of my travel was to inhabit, at least for several days, the urban environment that once contained the peripatetic musings of Søren Kierkegaard. I went to Denmark’
Leo Cabranes-Grant
doaj +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Actualism, Serious Actualism, and Quantified Modal Logic
Notre Dame Journal of Formal Logic, 2018 The paper navigates in the waters between metaphysical doctrine and formal logical representation. The goal is to build a first-order modal logic that accommodates both ``actualism'' (the view there are no merely possible things, that is, things that do not exist but have some alternative kind of being) and ``serious actualism'' (that all sentences ...
openaire +2 more sources
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
This paper gives a new account of the actuality entailments of ability claims. We observe that, in the environments which give rise to the actuality entailment, ability claims carry a presupposition of trying. We show that, given this presupposition, the
Schultheis, Ginger, Boylan, David
core +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
FEBS Open Bio, EarlyView.BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Modality and Actuality: Lukács’s Criticism of Hegel in History and Class Consciousness
Open PhilosophyThe present article tries to show the originality of Lukács’s theory of Actuality (Wirklichkeit) by comparing it with the same notion in Hegel’s Logic. It results that Lukács’s interpretation of Marxism and the Russian Revolution depends on a clear and ...
Rametta Gaetano
doaj +1 more source
HTS Teologiese Studies/Theological Studies, 2016 Virtuality in Dietrich Bonhoeffers’ Sanctorum Communio: Collective intelligence as a new epistemology of the church? Collective intelligence has been indicated from biological, philosophical, anthropological and technological developments.
Deon du Toit
doaj +1 more source