Results 171 to 180 of about 4,701,672 (287)
Quantifying microbial fitness in high-throughput experiments. [PDF]
Fink JW, Manhart M.
europepmc +1 more source
Evolution‐guided yeast complementation reveals functional differences in human PSPH variants
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez +6 more
wiley +1 more source
<sup>17</sup>O Quadrupole Coupling Constants in Water. [PDF]
Haque N, de Dios AG, de Dios AC.
europepmc +1 more source
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli +3 more
wiley +1 more source
Using simulation to disambiguate transducer and noise. [PDF]
Aguilar G, Maertens M, Vincent J.
europepmc +1 more source
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
Robust Disturbance Estimation-Based Control: Case Study on an Airship Attitude Tracking Problem. [PDF]
Stoica AM, Pană V, Ştefănescu IB.
europepmc +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Quantifying genetic load through joint modelling of inbreeding depression and inbreeding load for litter size in rabbits. [PDF]
Hervás-Rivero C +6 more
europepmc +1 more source

