Results 111 to 120 of about 2,556,175 (314)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Comparative Advantage: Theory, Empirical Measures And Case Studies [PDF]
This paper consists of three main parts i.e. theory, analytical tool and case studies of comparative advantage. Firstly, we review the theory and various empirical measures of comparative advantage. We would argue that for the catching-up economies, like
Tri WIDODO
core +1 more source
FEBS Open Bio, EarlyView.This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake +4 more
wiley +1 more source
“THE EFFICACY OF rTMS WITH PSYCHOTHERAPY, SLEEP MODIFICATIONS & EXERCISE.”
Brain Stimulation, 2020 Karan Narwal, William Hervey, M.D
doaj +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Competitive Advantage for Small and Medium Enterprises
Winning business is grounded in sustainable competitive advantage. A company has competitive advantage whenever it has on edge over rivals in securing customers and defending against competitive forces.
Ščeulovs, Deniss +1 more
core
China's Local Comparative Advantage [PDF]
China's trade pattern is influenced not just by its overall comparative advantage in labor intensive goods but also by geography. We use two variants of the Eaton-Kortum (2002) model to study China's local comparative advantage.
James Harrigan, Haiyan Deng
core
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Journal of Medicinal ChemistryDrug discovery is a complex, multi-parameter optimization process. I argue that a greater emphasis on optimizing binding affinity will accelerate the development of new medicines. Note that “optimizing” is not always synonymous with “maximizing.” While affinity is certainly not the only thing that matters, the value of optimizing drug – receptor ...
openaire +2 more sources
MiR‐513a promotes human erythroid differentiation by modulating c‐Jun
FEBS Open Bio, EarlyView.During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim +11 more
wiley +1 more source