FEBS Open Bio, EarlyView.This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel +4 more
wiley +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
EFFECTS OF SPOILER-SLOT-DEFLECTOR CONTROL ON THE AERODYNAMIC CHARACTERISTICS AT A MACH NUMBER OF 2.01 OF A VARIABLE-WINGSWEEP CONFIGURATION WITH THE OUTER WING PANELS SWEPT BACK 75 DEG [PDF]
Aerodynamic characteristics of variable-wing-sweep configuration with spoiler-slot-deflector control system & swept back outer wing ...
Foster, G. V.
core +1 more source
FEBS Open Bio, EarlyView.Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
Effect of SLD ice on the aerodynamic characteristics of supercritical airfoils. [PDF]
Sci ProgJie Y +5 more
europepmc +1 more source
AERODYNAMIC CHARACTERISTICS OF TWO RECTANGULAR-PLAN-FORM, ALLMOVABLE CONTROLS IN COMBINATION WITH A SLENDER BODY OF REVOLUTION AT MACH NUMBERS FROM 3.00 TO 6.25 [PDF]
Aerodynamic characteristics of rectangular planform, all-movable controls combined with slender body of revolution at mach 3 to 6 ...
Gloria, H. R., Wong, T. J.
core +1 more source
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
FEBS Open Bio, EarlyView.Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
Study of aerodynamic characteristics of variable cross-section box girders under three-dimensional fluctuating wind field. [PDF]
Sci RepFeng X, Jia J.
europepmc +1 more source
Results of tests in the MSFC 14 x 14 inch trisonic wind tunnel on a .004 scale model of the Rockwell International Space Shuttle Vehicle 3, (integrated configuration) [PDF]
Experimental aerodynamic investigations were conducted during mid-July, 1973 on a .004 scale model of the Rockwell International integrated configuration Space Shuttle Vehicle 3.
Allen, E. C., Hamiliton, T.
core +1 more source
Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis
FEBS Open Bio, EarlyView.Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho +4 more
wiley +1 more source