Results 161 to 170 of about 5,414,929 (281)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

De novo synthesis of short‐chain aldehydes and hydrocarbons secreted by the brown marmorated stink bug Halyomorpha halys

open access: yesFEBS Open Bio, EarlyView.
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Heterotropic regulation and negative homotropic cooperativity

open access: yesFEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea   +5 more
wiley   +1 more source

Evaluation of HYFLEX aerodynamic characteristics

open access: yesEvaluation of HYFLEX aerodynamic characteristics
24-25 Jul. 1996 24-25 Jul. 1996 HYFLEX(極超音速飛行実験)機の飛行実験において取得された空力特性データと風洞試験およびCFD(数値流体力学)に基づく予測値を比較することにより、HYFLEXにおいて使用した空力特性予測手法の妥当性を評価する。本稿が対象とするのは、空力係数、縦トリム特性、エレボン・ヒンジモーメントと機体表面圧力分布である。飛行試験と予測値の比較の結果、HYFLEXの空力設計において適用した空力特性予測手法は、全般的に妥当なものであることが検証された。しかし、軸力係数、トリム舵角などについては、予測値と飛行結果の間に設計で考慮したUncertainty以上の差異が見られたため、その原因について考察した。 HYFLEX (Hypersonic ...
openaire   +1 more source

Home - About - Disclaimer - Privacy