Results 101 to 110 of about 635,092 (276)

Measurement of Mutual Interference Sound of Columnar Objects in Air Flow

open access: yesMATEC Web of Conferences, 2018
When a columnar object is put in a flow, Karman vortex is formed, and aerodynamic noise is generated. It is known that when multiple columnar objects are put in a flow, the flow state becomes complex.
Hamada Shohji, Yokoi Yoshifumi
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Using cell‐free RNA to identify B‐ and T‐cell clonality for diagnosis and monitoring of B‐ and T‐cell neoplasms

open access: yesFEBS Open Bio, EarlyView.
Using peripheral blood for determining B‐cell or T‐cell clonality is more reliable when we use cell‐free RNA (cfRNA) because cells release blood significantly more RNA than DNA. Next‐generation sequencing (NGS) of cfRNA allows us to evaluate fragment cfRNA and evaluate clonality reliably without the need for prior determination of the specific dominant
Adam Albitar   +11 more
wiley   +1 more source

Computational Fluid Dynamic Simulation (CFD) and Experimental Study on Wing-external Store Aerodynamic Interference of a Subsonic Fighter Aircraft

open access: yesActa Polytechnica, 2003
The main objective of the present work is to study the effect of an external store on a subsonic fighter aircraft. Generally most modern fighter aircrafts are designed with an external store installation.
Tholudin Mat Lazim   +2 more
doaj  

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

open access: yesFEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source

Aerodynamic Shape Optimization Using the Discrete Adjoint of the Navier-Stokes Equations: Applications Toward Complex 3D Configutations [PDF]

open access: yes, 2009
Within the next few years, numerical shape optimization based on high fidelity methods is likely to play a strategic role in future aircraft design. In this context, suitable tools have to be developed for solving aerodynamic shape optimization problems,
Dwight, Richard P.   +5 more
core   +1 more source

Reducing Aerodynamic Interference Through Layout Optimization of Symmetrically Cambered Wingsails: A Comparative Study of In-Line and Parallel Configurations

open access: yesJournal of Marine Science and Engineering
Rigid wingsails are increasingly adopted for wind-assisted ship propulsion, with Symmetrically Cambered (SC) profiles identified as highly efficient for thrust generation.
Stephan van Reen   +5 more
doaj   +1 more source

Identification and characterization of a gene conferring stress resistance in Escherichia coli and cyanobacteria

open access: yesFEBS Open Bio, EarlyView.
Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada   +7 more
wiley   +1 more source

Open-Wheel Aerodynamics: Effects of Tyre Deformation and Internal Flow [PDF]

open access: yes, 2013
Competitive performance of an F1 race car relies upon a well designed and highly developed aerodynamic system. In order to achieve this, total understanding of the downstream wake of exposed rotating wheels is essential.
SPROT, ADAM,JOSEPH
core  

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