Results 41 to 50 of about 4,132,625 (266)

Dual‐line Genome‐scale CRISPR Screening Enables Robust Target Gene Discovery

Advanced Science, EarlyView.
A species‐optimized CRISPR platform integrates efficient piggyBac delivery, genome‐scale sgRNA libraries, and parallel screening in two independently engineered Bactrocera dorsalis Cas9 cell lines. Cross‐line consensus analysis filters line‐specific effects, enriches candidates with reproducible in vivo phenotypes, and reveals conserved, species ...
Ziniu Li, Jiao Qiao, Qiuyuan Zhang, Peipei Liu, Chenjun Zheng, Qi Zhou, Mengqi Ji, Zhihui Zhu, Xiaoxue Li, Hongyu Zhang   +9 more
wiley   +1 more source

Drought Stress Mediated Changes in Food Crops: Mechanisms and Remediation Strategies

Advanced Science, EarlyView.
This work presents a multi‐scale framework for flavonoid‐mediated drought tolerance in staple food crops, integrating ROS redox regulation, antioxidant defense, rhizosphere microbial interactions, and sustainable agronomic practices. This interdisciplinary model provides core insights to develop climate‐resilient cropping systems and enhance global ...
Xiaoyi Duan, Litao Wang, Tuuli‐Marjaana Koski, Lizhe An, Thomas Efferth, Yujie Fu   +5 more
wiley   +1 more source

Rainfall Shapes the Diversity of Soil Nitrogen‐Fixing Microorganisms Worldwide

Advanced Science, EarlyView.
This study reveals the distinctive pattern and mechanism of rainfall driving the global biodiversity and biogeography of soil potential N‐fixing microorganisms, and constructs the theoretical framework. The results guide us on how to maintain ecosystem productivity under future climate changes (e.g., whether a specific region needs more N fertilizers ...
Bin Hua, Shuang Pang, Ang Li, Zonghao Hu, Honghui Wu, Shuhan Zhang, Yi Fan, Yi Wu, Wei Yang, Yachao Zhao, Yupeng Guan, Baoming Ji, Deliang Kong, Yong Zhao, Anton A. Goncharov, Anastasia Yu. Korotkevich, Rong Mao, Yang Zhang, Ximei Zhang   +18 more
wiley   +1 more source

The Mitogen‐Activated Protein Kinase GhNTF3 Modulates the Arbuscular Mycorrhizal Symbiosis‐Immunity Trade‐Off in Cotton by Regulating Salicylic Acid Biosynthesis

Advanced Science, EarlyView.
AM symbiosis induces GhWAK13 to promote fungal colonization. GhNTF3 negatively regulates symbiosis by promoting SA biosynthesis. GhWAK13 sequesters GhNTF3 at the membrane, limiting its nuclear translocation and relieving SA‐mediated suppression. Under Verticillium stress, GhNTF3 enters the nucleus and interacts with GhJAZ6 to activate SA signaling ...
Shuangjie Jia, Jingshang Wen, Jihang Li, Meina Feng, Yiming He, Ruojun Liu, Qian Liu, Junli Zhang, Kai Cheng, Xiangyu Zhang, Xiao Zhang   +10 more
wiley   +1 more source

Short-term microbial community dynamics induced by 13C-labeled maize root, its derived biochar and NPK in long-term amended soil

Biochar
Crop residues and their derived biochar are frequently used for their potential to improve grain yield, soil fertility and carbon (C) sequestration. However, the effects of root are often overlooked, and the effects of chemical fertilizer (NPK) combined ...
Zonglin Lu, Tong Lu, Junmei Shi, Kun Chen, Hangming Guo, Na Li, Xiaori Han   +6 more
doaj   +1 more source

Functional–Taxonomic Scaling Resolves Conflicting Average Genome Size Estimates Across Environmental Gradients

Advanced Science, EarlyView.
Based on genome size‐functional breadth theory, we introduce and validate a framework that adjudicates conflicting average genome size (AGS) estimates by testing which estimate yields a gap between scaled functional diversity and scaled taxonomic diversity that is consistent with the expected positive association between genome size and gene functional
Huanhuan Zhu, Xiaoye Chen, Qiushi Li, Chenyu Sun, Haihua Xia, Yingxin Huang, Cong Wang, Peilin Chen, Cheng Gao   +8 more
wiley   +1 more source

Usage of Agricultural Land for Non-Agricultural Purposes in Slovakia

EU agrarian Law, 2018
The paper analyzes the usage of agricultural land for non-agricultural purposes in Slovakia, the historical background of usage and protection of agricultural land with emphasis on its withdrawal and administrative procedure.
Melišková Ina
doaj   +1 more source

Institutional Factors Affecting Agricultural Land Markets [PDF]

This paper analyses the main institutional factors affecting the rental and sales markets for agricultural land. Particular attention is paid to the effects of the common agricultural policy on land markets, and more specifically the underlying mechanism
Ciaian, Pavel,, Swinnen, Jo, Van Herck, Kristine, Kancs, d’Artis, Vranken, Liesbet   +4 more
core  

Antigen‐Detected NMR for Minimal Epitope Engineering and Structure‐Guided Selection of a NaV1.7‐Selective Nanobody

Advanced Science, EarlyView.
A minimal extracellular epitope from voltage‐gated sodium channel NaV1.7 was engineered into an isotope‐labelled antigen for antigen‐detected nuclear magnetic resonance (NMR). Together with AlphaFold2 (AF2), this enabled structure‐guided selection of R4C8, a human NaV1.7‐selective nanobody that labels fixed NaV1.7‐expressing cells and produces staining
Junyu Liu, Wanlin Chen, Ben Cristofori‐Armstrong, Theo Crawford, Kai‐En Chen, Peng Xie, Rainbow Wing Bo Chan, Yifei Zhu, Mimi Golder, Anneka Pereira Schmidt, Jennifer D. Naughton, Nicholas D. Condon, Åsa Andersson, Farhad Dehkhoda, Kirsten L. McMahon, Tabea Klasfauseweh, Ashvriya Thapa, Hue Tran, Poanna Tran, Sina Jami, Lotten Ragnarsson, Sebastian G. B. Furness, Jennifer R. Deuis, Brett M. Collins, Wai‐Hong Tham, Indira Prasadam, Irina Vetter, Mehdi Mobli   +27 more
wiley   +1 more source

Participatory evaluation and agronomic validation of Becium grandiflorum propagation techniques for sustainable honeybee forage development in the Wag-Himra Zone, northern Ethiopia

Discover Agriculture
Background Degraded natural forage limits honeybee productivity and threatens livelihoods in Ethiopia’s dryland. This study evaluated two propagation techniques for Becium grandiflorum seedling-in-pot (SP) and cutting-in-pot (CP) through participatory ...
Ertiban Desale, Yesuf Ibrahim, Agazhe Tsegaye, Ayalew Girmay, Meresa Lemma, Addisu Bihonegn, Alemu Tsegaye   +6 more
doaj   +1 more source