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Pea transformation: History, current status and challenges
This review recapitulates the history, important milestones, the current status, and the perspectives of the pea (Pisum sativum L.) transformation as a tool for pea crop breeding.
Michaela Ludvíková, Miroslav Griga
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The procedure for vacuum infiltration of cultivars A. caudatus L. and hybrids of A. caudatus L. xA. paniculatus L. was optimized. The functioning of gene construction pCBv19 in the Amaranthus leaves was evaluated by the transient expression after vacuum ...
Olha Mikolaivna Yaroshko +1 more
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TRA1: A Locus Responsible for Controlling Agrobacterium-Mediated Transformability in Barley
In barley (Hordeum vulgare L.), Agrobacterium-mediated transformation efficiency is highly dependent on genotype with very few cultivars being amenable to transformation.
Beata Orman-Ligeza +6 more
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Leaf disc transformation is one of the traditional methods that are now widely used in chrysanthemum with highly economical and ornamental value in world flower production, but it depends on plant genotypes and is time consuming and complicated.
Sujuan Xu +6 more
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A highly efficient genetic transformation system for broccoli and subcellular localization
IntroductionAgrobacterium-mediated genetic transformation has been widely used for the identification of functional genes and regulatory and developmental mechanisms in plants.
Yongyu Zhao +4 more
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Genetic transformation of wheat via Agrobacterium-mediated DNA delivery [PDF]
The method described involves an initial incubation of wheat immature embryos in a liquid culture of Agrobacterium tumefaciens. The Agrobacterium strain is engineered to contain a binary vector with a gene of interest and a selectable marker gene placed ...
Doherty, A. +5 more
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Transformation And Light Inducible Expression of cry1Ab Gene in Oilseed Rape (Brassica napus L.) [PDF]
Rapeseed (Brassica napus L.) is the third most important oil crop in global productions. One of the major limiting factors for oilseed rape production is lepidopteran pests of the Brassicaceae family.
H. Rahnama, M. Sheykhhasan
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Marker-free transgenic cucumber expressing Arabidopsis cbf1 gene confers chilling stress tolerance
Marker-free transgenic cucumber (Cucumis sativus L.) cv. Poinsett 76 SR plants were produced by Agrobacterium mediated transformation. A transformation efficiency of 1.62 was observed on using Agrobacterium tumefaciens strain LBA4404 harbouring ...
N. Gupta +6 more
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Approved genetically modified (GM) horticultural plants: A 25-year perspective
In this review, we present genetically modified (GM) horticultural events that have passed the regulatory process and have been approved for cultivation or food use in different countries.
Baranski Rafal +2 more
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Problems and possibilities of monocot transformation
Biotechnological improvement of monocots is often hampered by the lack of efficient regeneration systems, requisite wound responses and low cell competence. Despite these limitations, the biolistic and Agrobacterium methods have been successfully used to
P. Sood, A. Bhattacharya, A. Sood
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