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Agrobacterium molecular genetics
1988The bacteria of the genus Agrobacterium are well known as gene vectors for plant cells. In this chapter I shall discuss the following items: (l)the growth properties of different Agrobacterium strains; (2) methods for the introduction of DNA into Agrobacterium ; (3) rescue of plasmids via cointegration ; (4) gene replacement.
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The Agrobacterium Virulence System
1991The soil bacterium Agrobacterium tumefaciens causes the plant tumor crown gall at wound sites on dicotyledonous plants (Fig.1). During crown gall induction, agrobacteria transfer a segment of their tumor-inducing (Ti)-plasmid to plant cells at the wound sites.
Paul J. J. Hooykaas +3 more
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Cold Spring Harbor Protocols, 2006
INTRODUCTIONBecause transformation of Arabidopsis is a relatively long-winded process, it is a good idea to verify the presence of the T-DNA plasmid in the Agrobacterium strain before proceeding with transformation experiments. This protocol describes a method to determine the presence of plasmid DNA in an Agrobacterium culture.
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INTRODUCTIONBecause transformation of Arabidopsis is a relatively long-winded process, it is a good idea to verify the presence of the T-DNA plasmid in the Agrobacterium strain before proceeding with transformation experiments. This protocol describes a method to determine the presence of plasmid DNA in an Agrobacterium culture.
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Plant-Agrobacterium Interaction [PDF]
, 1986 The plant-Agrobacterium interaction is a well-studied example of cell to cell signaling. It is giving us important information about factors and processes which play a role in pathogenesis.
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1991
El género Agrobacterium pertenece a la familia Rhizobiaceae Conn 1938, que comprende también los géneros Rhizobium, Bradyrhizobium y Phyllobacterium. Kersters y De Ley (1984) en la novena edición del Bergey's Manual señalan que según las normas de taxonomía bacteriana deben considerarse cuatro especies en el género Agrobacterium: A.
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El género Agrobacterium pertenece a la familia Rhizobiaceae Conn 1938, que comprende también los géneros Rhizobium, Bradyrhizobium y Phyllobacterium. Kersters y De Ley (1984) en la novena edición del Bergey's Manual señalan que según las normas de taxonomía bacteriana deben considerarse cuatro especies en el género Agrobacterium: A.
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Exploitation of Agrobacterium tumefaciens
1992During invasion of wounded plants, soil agrobacteria transfer a defined segment of their Ti and Ri plasmids into the plants. The transferred DNA, termed T-DNA, is integrated into the plant nuclear genome. Genes encoded by Ti and Ri plasmid T-DNAs are expressed in plants and confer the synthesis of plant growth factors as well as sugar and amino acid ...
Jozef Schell, Csaba Koncz
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Plant cyclophilins and Agrobacterium
Trends in Plant Science, 1998Abstract Deng, W., Chen, L., Wood, D.W., Metcalfe, T., Liang, X., Gordon, M.P., Comai, L. and Nester, E.W. (1998) Agrobacterium VirD2 protein interacts with plant host cyclophilins, Proc. Natl. Acad. Sci. U. S. A .
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pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation
Plant Molecular Biology, 2000R. Hellens +4 more
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Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method
Nature Protocols, 2006Xiuren Zhang +4 more
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