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PCR Analysis of Agrobacterium

Cold Spring Harbor Protocols, 2006
INTRODUCTIONBecause transformation of Arabidopsis is a relatively long-winded process, it is a good idea to verify the presence of the T-DNA plasmid in the Agrobacterium strain before proceeding with transformation experiments. This protocol describes a method to determine the presence of plasmid DNA in an Agrobacterium culture.
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Género Agrobacterium

1991
El género Agrobacterium pertenece a la familia Rhizobiaceae Conn 1938, que comprende también los géneros Rhizobium, Bradyrhizobium y Phyllobacterium. Kersters y De Ley (1984) en la novena edición del Bergey's Manual señalan que según las normas de taxonomía bacteriana deben considerarse cuatro especies en el género Agrobacterium: A.
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pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation

Plant Molecular Biology, 2000
R. Hellens   +4 more
semanticscholar   +1 more source

Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method

Nature Protocols, 2006
Xiuren Zhang   +4 more
semanticscholar   +1 more source

Agrobacterium

2013
B. Lacroix, V. Citovsky
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Agrobacterium tumefaciens-Mediated Transformation of Tomato.

Methods in molecular biology, 2018
J. Van Eck   +2 more
semanticscholar   +1 more source

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