Experiments were conducted to establish an efficient and simple protocol for regeneration and Agrobacterium mediated genetic transformation of an agronomically important indica rice variety Bg 250.
RMLK Ratnayake, GHCM Hettiarachchi
doaj +1 more source
Studies on plant gene transfer systems [PDF]
A number of methods for the transfer of genes to plants are assessed in this work. The potential of Agrobacterium tumefaciens infection of germinating pollen tubes in vitro as a method of gene transfer was investigated and evidence presented for an ...
Sherman, Adrian
core
Review of methodologies and a protocol for the
Since the first report of wheat transformation by Agrobacterium tumefaciens in 1997, various factors that influence T-DNA delivery and regeneration in tissue culture have been further investigated and modified.
Doherty, A. +5 more
core +1 more source
Transposase‐Assisted Donor Tethering Boosts Large‐Fragment HDR in Plants
A transposase‐assisted donor tethering strategy is developed to enhance homology‐directed repair in plants. By recruiting donor DNA to double‐strand breaks and synergizing with repair pathway reprogramming and transcription‐coupled donor design, this system markedly improves large‐fragment targeted insertion efficiency, providing a robust platform for ...
Sha Wei +8 more
wiley +1 more source
GENETICALLY MODIFIED FRUITS: THE SOLUTION OR A PROBLEM? [GENETICKY MODIFIKOVANÉ OVOCE: ŘEŠENÍ NEBO PROBLÉM?] [PDF]
Genetically modified (GM) fruit crops breeding began in the 1990s and brought several successful GM varieties. The first successful GM fruit tree was a transgenic papaya, which saved its growing industry in Hawaii.
Michaela Burešová
doaj +1 more source
TRANSIENT EXPRESSION OF REPORTER GENES IN CULTIVARS OF Amaranthus caudatus L. [PDF]
Local cultivars of A. caudatus: Helios and Karmin were used as plant material. Amaranth is a new pseudocereal introduced in Ukraine. The plant biomass of amaranth is used in medicine, food industry and cosmetology industry. Aim.
O. M. Yaroshko, M. V. Kuchuk
doaj
NLSS3 Impairs SHM1 Autophagic Degradation to Regulate Leaf Morphology and Salt Tolerance in Rice
In rice, NLSS3 binds and shields SHM1 from autophagic degradation to maintain serine homeostasis. The A132P mutation in nlss3 disrupts this, triggering SHM1 loss, metabolic dysfunction, K+ imbalance, and impaired ROS scavenging, which collectively drive narrow leaves and salt sensitivity.
Xiong Liu +15 more
wiley +1 more source
Improved genome of Agrobacterium radiobacter type strain provides new taxonomic insight into Agrobacterium genomospecies 4 [PDF]
The reported Agrobacterium radiobacter DSM 30174T genome is highly fragmented, hindering robust comparative genomics and genome-based taxonomic analysis. We re-sequenced the Agrobacterium radiobacter type strain, generating a dramatically improved genome
Han Ming Gan (7214096) +2 more
core +2 more sources
Attachment of Agrobacterium to plant surfaces [PDF]
Agrobacterium tumefaciens binds to the surfaces of inanimate objects, plants, and fungi. These bacteria are excellent colonizers of root surfaces. In addition, they also bind to soil particles and to the surface of artificial or man-made substances, such as polyesters and plastics.
openaire +3 more sources
Identification and analysis of a flagellar gene operon of Agrobacterium tumefaciens [PDF]
The cosmid, pDUB1900, from a representative genomic library of the Agrobacterium tumefaciens C58C1 chromosome has previously been partly mapped and found to contain genes concerned with flagellar structure, assembly and function.
Kelly, Victoria Elizabeth
core

