Effects of various Agrobacterium rhizogenes strains on hairy root induction and analyses of primary and secondary metabolites in Ocimum basilicum [PDF]
Frontiers in Plant Science, 2022 The hairy root (HR) culture system is an excellent alternative strategy to the whole plant system for producing valuable compounds. However, selection of suitable Agrobacterium strain for the successful induction of HR is an essential step for enhanced ...
Ramaraj Sathasivam +2 more
exaly +3 more sources
Highly efficient Agrobacterium rhizogenes-mediated hairy root transformation for gene functional and gene editing analysis in soybean [PDF]
Plant Methods, 2021 Background Agrobacterium-mediated genetic transformation is a widely used and efficient technique for gene functional research in crop breeding and plant biology.
Yuanyuan Cheng +5 more
doaj +3 more sources
Agrobacterium rhizogenes—mediated transformation of Pisum sativum L. roots as a tool for studying the mycorrhizal and root nodule symbioses [PDF]
PeerJ, 2019 In this study, we demonstrated the successful transformation of two pea (Pisum sativum L.) cultivars using Agrobacterium rhizogenes, whereby transgenic roots in the resulting composite plants showed expression of the gene encoding the green fluorescent ...
Irina V. Leppyanen +2 more
doaj +4 more sources
Agrobacterium rhizogenes: paving the road to research and breeding for woody plants. [PDF]
Front Plant Sci, 2023 Woody plants play a vital role in global ecosystems and serve as valuable resources for various industries and human needs. While many woody plant genomes have been fully sequenced, gene function research and biotechnological breeding advances have ...
Ying W +12 more
europepmc +2 more sources
Frontiers in Microbiology, 2016 The development of an efficient protocol for successful hairy root induction by Agrobacterium rhizogenes is the key step toward an in vitro culturing method for the mass production of secondary metabolites.
Mariadhas Valan Arasu +2 more
exaly +4 more sources
Highly efficient Agrobacterium rhizogenes-mediated transformation for functional analysis in woodland strawberry. [PDF]
Plant Methods, 2023 Background The diploid woodland strawberry ( Fragaria vesca ) is an excellent model plant for investigating economically significant traits and several genetic resources within the Rosaceae family.
Yan H +6 more
europepmc +2 more sources
Highly efficient Agrobacterium rhizogenes-mediated hairy root transformation in citrus seeds and its application in gene functional analysis. [PDF]
Front Plant Sci, 2023 Highly efficient genetic transformation technology is beneficial for plant gene functional research and molecular improvement breeding. However, the most commonly used Agrobacterium tumefaciens-mediated genetic transformation technology is time-consuming
Wang M, Qin YY, Wei NN, Xue HY, Dai WS.
europepmc +2 more sources
Optimization of in vitro and ex vitro Agrobacterium rhizogenes-mediated hairy root transformation of soybean for visual screening of transformants using RUBY. [PDF]
Front Plant Sci, 2023 In vitro and ex vitro Agrobacterium rhizogenes-mediated hairy root transformation (HRT) assays are key components of the plant biotechnology and functional genomics toolkit.
Niazian M +4 more
europepmc +2 more sources
Highly efficient Agrobacterium rhizogenes-mediated hairy root transformation for gene editing analysis in cotton. [PDF]
Front Plant Sci, 2022 CRIPSR/Cas9 gene editing system is an effective tool for genome modification in plants. Multiple target sites are usually designed and the effective target sites are selected for editing.
Zhou L +6 more
europepmc +2 more sources
Agrobacterium rhizogenes-mediated hairy root transformation as an efficient system for gene function analysis in Litchi chinensis. [PDF]
Plant Methods, 2021 Litchi chinensis Sonn. is an economically important fruit tree in tropical and subtropical regions. However, litchi functional genomics is severely hindered due to its recalcitrance to regeneration and stable transformation.
Qin Y +6 more
europepmc +2 more sources