Results 31 to 40 of about 229,976 (265)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Scythe AI: A Tool for Modular Reuse of Game AI

open access: yesProceedings of the AAAI Conference on Artificial Intelligence and Interactive Digital Entertainment, 2013
Scythe AI is a tool designed for game AI developers to enable modular reuse of NPC AI. In this demonstration, we show how Scythe AI can be used to compose and manage statechart-based AI-Modules to produce new AIs for a target game. The tool includes a streamlined importing process to introduce new modules, library management of modules, an error and ...
Christopher Dragert   +2 more
openaire   +2 more sources

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

open access: yesFEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel   +4 more
wiley   +1 more source

Navigating ChatGPT for Business English Writing: Benefits, Limitations, and Challenges for Students [PDF]

open access: yesOvidius University Annals: Economic Sciences Series
The present study explores the use of ChatGPT as a tool for enhancing Business English (BE) writing skills among first-year students in a Finance and Banking program.
Eleonora Baca
doaj  

The Use of Artificial Intelligence among Students in Higher Education

open access: yesOrganizacija
Artificial intelligence (AI) impacts our everyday lives, from business to social areas, and, in recent years, more and more education. This paper aims to discuss using artificial intelligence tools for educational purposes from the student’s perspective.
Jereb Eva, Urh Marko
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

open access: yesFEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková   +3 more
wiley   +1 more source

Ethical guidelines for the use of generative artificial intelligence and artificial intelligence-assisted tools in scholarly publishing: a thematic analysis [PDF]

open access: yesScience Editing
Purpose This analysis aims to propose guidelines for artificial intelligence (AI) research ethics in scientific publications, intending to inform publishers and academic institutional policies in order to guide them toward a coherent and consistent ...
Adéle da Veiga
doaj   +1 more source

Combined 5‐aminolevulinic acid and ferric ammonium citrate treatment promotes hair follicle growth by activating dermal papilla cells

open access: yesFEBS Open Bio, EarlyView.
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley   +1 more source

The role of neural networks in the development of communicative competence of technical science students

open access: yesВестник Самарского государственного технического университета. Серия: Психолого-педагогические науки
Communicative competence has a significant impact on the successful integration of a modern specialist into the professional environment, so its development is one of the priority tasks of educational institutions.
Yulia V. Troitskaya
doaj   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

open access: yesFEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke   +4 more
wiley   +1 more source

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