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Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Exploring the Relationship Between Comorbidities and Prolonged Viral Shedding in COVID-19: A Cycle Threshold Value-Based Investigation. [PDF]

open access: yesCan J Infect Dis Med Microbiol
Wisaksana R   +8 more
europepmc   +1 more source

Strengthening HIV Programs in Sub-Saharan Africa Through Implementation Research: A Mixed-Methods Systematic Review. [PDF]

open access: yesAIDS Res Treat
Bwire GM   +14 more
europepmc   +1 more source
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Does AID aid AIDS?

Immunology Letters, 2004
During AIDS, the acquisition of mutations in the HIV-1 gp120 envelope glycoprotein leads to the switch from primary R5 (CCR5-using) to highly cytopathic X4 (CXCR4-using) HIV-1 variants. Based on the already known sequence homology between IgV genes and the gp120-coding region of the env gene, as well as on somatic hypermutation of multiple proto ...
openaire   +2 more sources

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