Results 61 to 70 of about 1,779,598 (269)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Investigating SARS-CoV-2 Virus in Environmental Surface, Water, Wastewater and Air: A Systematic Review [PDF]
Health in Emergencies & Disasters QuarterlyBackground: The occurrence of COVID-19 as a public health emergency of international alarm was declared by the World Health Organization (WHO) on January 30, 2020.
Parisa Javanbakht +4 more
doaj
FEBS Open Bio, EarlyView.Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
Elaboration of High Permeable Macrovoid Free Polysulfone Hollow Fiber Membranes for Air Separation
Fibers, 2019 In this work, polysulfone hollow fibers with oxygen permeance 70 L (STP)/(m2·h·bar) and selectivity α(O2/N2) = 6 were obtained. A decrease in the dope solution temperature allowed to diminish macrovoids due to the increase of the dope ...
George Dibrov +5 more
doaj +1 more source
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
FEBS Open Bio, EarlyView.Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
Microorganisms in stable air as possible postsecretory milk contaminants
Mljekarstvo, 2006 Quality and hygienic correct milk must satisfy conditions which are prescribed by Law on Raw Milk Quality (NN102/2000). If we know that freshly milked milk could contain a certain number of microorganisms originating from the stable air (so called ...
Kristina Matković +4 more
doaj
A Commentary: Breathing Together Now
Engaging Science, Technology, and Society, 2020 It is a challenging and important time to breathe together. A reflection on the timeliness and resonance of the thematic collection "Breathing Late Industrialism."
Timothy Choy
doaj +1 more source
Acute caffeine treatment protects the developing retina from ischemia‐induced cell death
FEBS Open Bio, EarlyView.Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento +6 more
wiley +1 more source
FEBS Open Bio, EarlyView.Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero +5 more
wiley +1 more source