Cloning and expression of a thermostable α-galactosidase from the thermophilic fungus Talaromyces emersonii in the methylotrophic yeast Pichia pastoris [PDF]
The first gene (alpha-gal1) encoding an extracellular alpha-Dgalactosidase from the thermophilic fungus Talaromyces emersonii was cloned and characterized.
Fernandes, Sara +4 more
core +1 more source
Glycomimetics with triazoline and aziridine groups were synthesised via diastereoselective Huisgen cycloaddition from glycosyl azides. Density functional theory was applied to define the mechanism for BF3 promoted aziridine formation. Triazoline and aziridine derivatives were synthesised by diastereo‐ and regioselective Huisgen cycloaddition of 2,3,4,6‐
Aaron McCormack +4 more
wiley +1 more source
A novel bio-functional material based on mammalian cell aggresomes [PDF]
Aggresomes are protein aggregates found in mammalian cells when the intracellular protein degradation machinery is over-titered. Despite that they abound in cells producing recombinant proteins of biomedical and biotechnological interest, the ...
Abasolo, Ibane +7 more
core +2 more sources
A Practical and Flexible De Novo Synthesis of Deoxygenated Carbasugars and Their Cyclitol Epoxides
A de novo synthesis route comprising enantioselective Brown crotylation and ring‐closing metathesis as the key steps enables the synthesis of a‐ and b‐carba‐paratose, a‐ and b‐carba‐colitose as well as four configurational deoxycyclophellitol isosteres.
Yevhenii Radchenko +4 more
wiley +1 more source
In vitro screening of probiotic lactic acid bacteria and prebiotic glucooligosaccharides to select effective synbiotics [PDF]
Probiotics and prebiotics have been demonstrated to positively modulate the intestinal microflora and could promote host health. Although some studies have been performed on combinations of probiotics and prebiotics, constituting synbiotics, results on ...
Courtin, Céline +6 more
core +2 more sources
Characteristic features of an alpha-galactosidase from mung beans [PDF]
Two molecular forms, I and II (high and low molecular mass) of alpha-galactosidase were demonstrated in dry mung beans and a multi-step procedure was developed for isolating the tetrameric enzyme I in good yield. Two affinity chromatographic techniques were employed and an overall 10 000-fold purification was achieved.
openaire +2 more sources
Recent Progress on Enzyme Immobilization: Materials, Strategies, and Applications
Enzyme immobilization technology has undergone substantial evolution driven by advances in materials science. This review systematically examines the recent advances in enzyme immobilization since 2024, with a focused analysis of five major categories of enzyme carriers including covalent organic framework, metal‐organic frameworks, polymers, carbon ...
Shuran Wang +7 more
wiley +1 more source
Case report: is low α-Gal enzyme activity sufficient to establish the diagnosis of Fabry disease?
Fabry disease is an X-linked lysosomal storage disease due to alpha-galactosidase A (α-Gal A) deficient activity which leads to the accumulation of glucoesphingolipids, such as globotriaosilceramide. There are over 700 known mutations of the enzyme gene,
Gilson Biagini +7 more
doaj +1 more source
Nonthermal Food Processing Technologies: A Comprehensive Review
The graphical abstract illustrates the shift from traditional thermal food preservation methods to advanced nonthermal technologies. Traditional methods, represented by heating, often compromise the nutritional and sensory quality of foods. In contrast, nonthermal approaches such as high‐pressure processing (HPP), pulsed electric field (PEF ...
Ashok Kumar Yadav +5 more
wiley +1 more source
Present status on removal of raffinose family oligosaccharides - a Review
Raffinose family oligosaccharides (RFOs) are α-galactosyl derivatives of sucrose or glucose. They are found in a large variety of seeds from many different families such as beans, vegetables and whole grains.
Jian Zhang +5 more
doaj +1 more source

