SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Direct RNA sequencing reveals multilayered epitranscriptomic remodeling in macrophages upon Mtb infection. [PDF]
Zou RC +9 more
europepmc +1 more source
Naked cuticle is essential for Drosophila wing development beyond Wingless signaling
Naked cuticle (Nkd), a Wnt signaling inhibitor, assumes extensive roles in Drosophila wing development. Overexpressing Nkd causes smaller, crumpled wings, while also perturbing multiple signaling pathways and developmental genes. A specific region (R1S) is critical for Nkd's function as a signaling integrator, offering new insights for studying its ...
Rui Wang, Ping Wang
wiley +1 more source
<i>In vitro</i> characterization of antimicrobial efficacy and cytotoxicity of polyvinylpyrrolidone-iodine, N-acetylcysteine, methylglyoxal, and N-chlorotaurine as alternative antimicrobials in treating bovine clinical endometritis. [PDF]
Pas D, Oltmanns H, Meißner J.
europepmc +1 more source
LAPTM5 drives omental metastasis in high-grade serous ovarian cancer via TGF-β/Smad-mediated epithelial plasticity. [PDF]
Gao Y +8 more
europepmc +1 more source
Prevalence and factors associated with the use of complementary and alternative medicine among PCOS patients. [PDF]
Ya'u DS +4 more
europepmc +1 more source
Microbial alternatives for pest control
Ciro Pedro Guidotti Pinto +1 more
openaire +1 more source
Beyond Bonferroni: new multiple contrast tests for time-to-event data under non-proportional hazards. [PDF]
Dormuth I +5 more
europepmc +1 more source

