Results 121 to 130 of about 600 (173)

Amanitin binding to RNA polymerase II in α-amanitin-resistant rat myoblast mutants

Journal of Molecular Biology, 1977
A series of independent α-amanitin-resistant (Ama R ) mutants have been isolated from diploid and tetraploid strains of L6 rat myoblast cells. With one exception, these mutants contain both α-amanitin-sensitive wild-type and α-amanitin-resistant mutant RNA polymerase II activities.
M M, Crerar, S J, Andrews, E S, David, D G, Somers, J L, Mandel, M L, Pearson   +5 more
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Amanitins in virus research

Archives of Virology, 1978
The advances in virus research made possible by the use of amanitins are reported; they include i) determination of the genetic source (i.e. cellular or viral) of the RNA polymerase transcribing the viral genome, and ii) mechanism of virus-induced shut-off of host RNA synthesis.
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α-Amanitin: Inactivation by bovine lactoperoxidase

Experientia, 1979
The principle amatoxin, alpha-amanitin, is found to be extremely sensitive toward lactoperoxidase catalyzed degradation, rather than iodination, of the indole nucleus. Extensive attenuation of inhibitor potency against eukaryotic DNA-dependent RNA polymerase II accompanies the treatment of alpha-amanitin with lactoperoxidase, iodide and hydrogen ...
P W, Morris, K M, Kelley, W G, Logas
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Clustered α-amanitin resistance mutations in mouse

Molecular and General Genetics MGG, 1995
We report the identification of three new alpha-amanitin resistance mutations in the gene encoding the largest subunit of mouse RNA polymerase II (RPII215). These mutations are clustered in a region of the largest subunit that is important for transcription elongation.
M S, Bartolomei, J L, Corden
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Antidotes to Experimental cc-Amanitin Poisoning

Nature New Biology, 1972
THE two most important toxic cyclopeptides isolated from the mushroom Amanita phalloides (death cap) are phalloidin and α-amanitin1, 2. The latter is considered to be responsible for the fatal cases of poisoning. Up to now, protection has been demonstrated either with a non-toxic chemical analogue (antamanide) isolated from the toadstool itself and ...
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MALDI-TOF mass spectrometric analysis of α-amanitin, β-amanitin, and phalloidin in urine

Forensic Toxicology, 2012
A rapid and sensitive method was developed for analysis of α-amanitin, β-amanitin, and phalloidin by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). In this method, α-cyano-4-hydroxy cinnamic acid was used as the matrix to assist the ionization of toxins.
Kunio Gonmori, Kayoko Minakata, Masako Suzuki, Itaru Yamagishi, Hideki Nozawa, Koutaro Hasegawa, Amin Wurita, Kanako Watanabe, Osamu Suzuki   +8 more
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Total Synthesis of α‐ and β‐Amanitin

Angewandte Chemie International Edition, 2020
Abstractα‐Amanitin and related amatoxins have been studied for more than six decades mostly by isolation from death cap mushrooms. The total synthesis, however, remained challenging due to unique structural features. α‐Amanitin is a potent inhibitor of RNA polymerase II. Interrupting the basic transcription processes of eukaryotes leads to apoptosis of
Christian Lutz, Werner Simon, Susanne Werner‐Simon, Andreas Pahl, Christoph Müller   +4 more
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Determination of α-amanitin and β-amanitin in human biological fluids by high-performance liquid chromatography

Analytical Biochemistry, 1985
A high-performance liquid chromatographic assay of alpha-amanitin and beta-amanitin in human serum, urine, or stomach washings is described. Sample preparation involves a chemical step with deproteinization and organic solvent treatment, and a selective cleanup and concentration step on reversed-phase prepacked cartridges.
F, Jehl, C, Gallion, P, Birckel, A, Jaeger, F, Flesch, R, Minck   +5 more
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Abstract 735: SAR of amanitin and optimization of linker-amanitin derivatives for solid tumors

Cancer Research, 2018
Abstract Background: ATACs (Antibody Targeted Amanitin Conjugates) comprise a new class of antibody-drug conjugates using amanitin as toxic payload. Amanitin binds to the eukaryotic RNA pol II and thereby inhibits the cellular transcription process at very low concentrations.
Michael Kulke, Anikó Pálfi, Christoph Müller, Werner Simon, Susanne Werner-Simon, Christian Lutz, Torsten Hechler, Andreas Pahl   +7 more
openaire   +1 more source

Viroid replication is inhibited by α-amanitin

Nature, 1979
VIROIDS are infectious single-stranded circular RNA molecules1 which cause disease in higher Plants. Although the complete molecular structure of one viroid has recently been established2, the mechanism of viroid replication and patho-genesis is still unknown.
H P, Mühlbach, H L, Sänger
openaire   +2 more sources