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Leveraging community engagement and human-centered design to develop multilevel implementation strategies to enhance adoption of a health equity intervention. [PDF]

Implement Sci Commun
Price MA, Mulkern PJ, Condon M, Rakhilin M, Johansen K, Lyon AR, Saldana L, Pachankis J, Woodward SA, Roeder KM, Moran LR, Jerskey BA.   +11 more
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Chestnut blight and recovering American chestnut trees in Michigan

Canadian Journal of Botany, 1983
Stands of American chestnut trees have survived in Michigan in spite of the presence of Endothia parasitica. Cankered trees were evaluated in eight groves in seven of which two types of abnormal cankers were observed. These abnormal cankers resulted when diseased periderm was replaced by wound periderm.
D. W. Fulbright, W. H. Weidlich, K. Z. Haufler, C. S. Thomas, C. P. Paul   +4 more
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American Chestnut— Castanea dentata

2023
This chapter recounts how the American chestnut once occupied a significant portion of many forests, yielding food, timber, and structure for people and wildlife. The loss of the American chestnut set the stage for much of the forest structure America has today.
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The American Chestnut's Genetic Rebirth

Scientific American, 2014
The article discusses genetic engineering efforts in the conservation of the American chestnut tree, focusing on work by the authors and others to develop fungus-resistant "transgenic" trees. Topics include the cryphonectria parasitica fungus epidemic, techniques aimed at reviving the American chestnut including hybridization with the Chinese chestnut ...
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Somatic embryogenesis in American chestnut

Canadian Journal of Forest Research, 1991
Cultures were initiated from developing ovules and excised embryos of American chestnut (Castaneadentata (Marsh.) Borkh.), collected from five source trees on three dates during early and middle stages of fruit development. Explants were cultured initially on semisolid induction medium containing 0.25 mg/L benzyladenine and either 6 mg/L ...
S. A. Merkle, A. T. Wiecko, B. A. Watson-Pauley   +2 more
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American Chestnut [Castanea dentata (Marsh.) Borkh.]

2006
The key to successful transformation of American chestnut is having the correct combination of explant tissue, selectable and scorable markers, and a reliable regeneration system. Rapidly dividing somatic embryos, growing as proembryogenic masses, are a suitable tissue; the bar gene is a suitable selectable marker in conjunction with 1.0 to 10 mg/L ...
Charles A, Maynard, Linda D, Polin, Sharon L, LaPierre, Ronald E, Rothrock, William A, Powell   +4 more
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Chestnut, American (Castanea dentata (Marsh.) Borkh.)

2014
The key to successful transformation of American chestnut is having the correct combination of explant tissue, selectable markers, a very robust DNA delivery system, and a reliable regeneration system. The most important components of this transformation protocol for American chestnut are the following: starting out with rapidly dividing somatic ...
Charles A, Maynard, Linda D, McGuigan, Allison D, Oakes, Bo, Zhang, Andrew E, Newhouse, Lilibeth C, Northern, Allison M, Chartrand, Logan R, Will, Kathleen M, Baier, William A, Powell   +9 more
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Enhancement of American chestnut somatic seedling production

Plant Cell Reports, 2005
Somatic embryogenesis holds promise for mass propagation of American chestnut trees bred or genetically engineered for resistance to chestnut blight. However, low germination frequency of chestnut somatic embryos has limited somatic seedling production for this forest tree.
G M, Andrade, S A, Merkle
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