SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Widely tunable and narrow-linewidth violet lasers enabled by UV-transparent materials. [PDF]
Franken CAA +12 more
europepmc +1 more source
Amplifier‐Based Programmable Metasurface for Bidirectional Full Polarization Enhancement
Xin Wang +5 more
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Autophagosome marker, LC3, is released extracellularly via several distinct pathways
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito +3 more
wiley +1 more source
Two-Color Fluorescence Thermometry Using Lock-in Amplifiers for Background Suppression. [PDF]
Anderson BR, Eilers H.
europepmc +1 more source
Design of a Microwave Bias-Tee Using Lumped Elements with a Wideband Characteristic for a High Power Amplifier [PDF]
Hyun-Seok Oh +2 more
openalex +1 more source
ATP13A2 is involved in intracellular polyamine transport in lung epithelial cells
Spermidine transport in lung epithelial cells involves the polyamine transporter ATP13A2. Cell proliferation is associated with the upregulation of ATP13A2. Polyamines are present in all living cells and are implicated in various crucial cellular processes such as proliferation, apoptosis and autophagy.
Yuta Hatori +8 more
wiley +1 more source
Design of low power energy efficient sigma-delta ADC for biomedical IoT applications. [PDF]
Sharma BP +4 more
europepmc +1 more source
Asymmetric optical amplifier based on PT-symmetry [PDF]
Rujiang Li, Pengfei Li, Lu Li
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